Phage display screening of bovine antibodies to foot-and-mouth disease virus and their application in a competitive ELISA for serodiagnosis

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dc.contributor.authorSukyo Jeong-
dc.contributor.authorHyun Joo Ahn-
dc.contributor.authorKyung-Jin Min-
dc.contributor.authorJ W Byun-
dc.contributor.authorH M Pyo-
dc.contributor.authorM Y Park-
dc.contributor.authorB KKu-
dc.contributor.authorJ Nah-
dc.contributor.authorS Ryoo-
dc.contributor.authorS H Wee-
dc.contributor.authorSang Jick Kim-
dc.date.accessioned2021-05-04T03:30:53Z-
dc.date.available2021-05-04T03:30:53Z-
dc.date.issued2021-
dc.identifier.issn1661-6596-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24290-
dc.description.abstractFor serodiagnosis of foot-and-mouth disease virus (FMDV), monoclonal antibody (MAb)-based competitive ELISA (cELISA) is commonly used since it allows simple and reproducible detection of antibody response to FMDV. However, the use of mouse-origin MAb as a detection reagent is questionable, as antibody responses to FMDV in mice may differ in epitope structure and preference from those in natural hosts such as cattle and pigs. To take advantage of natural host-derived antibodies, a phage-displayed scFv library was constructed from FMDV-immune cattle and subjected to two separate pannings against inactivated FMDV type O and A. Subsequent ELISA screening revealed high-affinity scFv antibodies specific to a serotype (O or A) as well as those with pan-serotype specificity. When BvO17, an scFv antibody specific to FMDV type O, was tested as a detection reagent in cELISA, it successfully detected FMDV type O antibodies for both serum samples from vaccinated cattle and virus-challenged pigs with even higher sensitivity than a mouse MAb-based commercial FMDV type O antibody detection kit. These results demonstrate the feasibility of using natural host-derived antibodies such as bovine scFv instead of mouse MAb in cELISA for serological detection of antibody response to FMDV in the susceptible animals.-
dc.publisherMDPI-
dc.titlePhage display screening of bovine antibodies to foot-and-mouth disease virus and their application in a competitive ELISA for serodiagnosis-
dc.title.alternativePhage display screening of bovine antibodies to foot-and-mouth disease virus and their application in a competitive ELISA for serodiagnosis-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Sciences-
dc.citation.number9-
dc.citation.endPage4328-
dc.citation.startPage4328-
dc.citation.volume22-
dc.contributor.affiliatedAuthorSukyo Jeong-
dc.contributor.affiliatedAuthorHyun Joo Ahn-
dc.contributor.affiliatedAuthorKyung-Jin Min-
dc.contributor.affiliatedAuthorSang Jick Kim-
dc.contributor.alternativeName정수교-
dc.contributor.alternativeName안현주-
dc.contributor.alternativeName민경진-
dc.contributor.alternativeName변재원-
dc.contributor.alternativeName표현미-
dc.contributor.alternativeName박미영-
dc.contributor.alternativeName구복경-
dc.contributor.alternativeName나진주-
dc.contributor.alternativeName류수윤-
dc.contributor.alternativeName위성환-
dc.contributor.alternativeName김상직-
dc.identifier.bibliographicCitationInternational Journal of Molecular Sciences, vol. 22, no. 9, pp. 4328-4328-
dc.identifier.doi10.3390/ijms22094328-
dc.subject.keywordFoot-and-mouth disease virus-
dc.subject.keywordType O-
dc.subject.keywordType A-
dc.subject.keywordPhage display-
dc.subject.keywordantibody-
dc.subject.keywordCompetitive ELISA-
dc.subject.localFoot-and-mouth disease virus (FMDV)-
dc.subject.localfoot-and-mouth disease virus-
dc.subject.localFoot-and-mouth disease virus-
dc.subject.localType O-
dc.subject.localtype A-
dc.subject.localType A-
dc.subject.localPhage display-
dc.subject.localphage display-
dc.subject.localantibody-
dc.subject.localAntibody-
dc.subject.localAntibodies-
dc.subject.localCompetitive ELISA-
dc.subject.localcompetitive ELISA-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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