Pollen-mediated flow of bar gene in transgenic herbicide-resistant turf grass Zoysia japonica

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Title
Pollen-mediated flow of bar gene in transgenic herbicide-resistant turf grass Zoysia japonica
Author(s)
H G Kang; O C Chung; T W Bae; H J Sun; In Ja Song; K W Park; P O Lim; J Lee; Y E Lee; P S Song; H Y Lee
Bibliographic Citation
Plant Biotechnology Reports, vol. 15, no. 2, pp. 241-250
Publication Year
2021
Abstract
Weed control can be most effectively achieved through the use of herbicide-resistance transgene. A preliminary study bar-transgenic Zoysia japonica posed no serious risk on the unintended escape of the transgene from its cultivation site. The present follow-up investigation of the dispersion of pollen and its short-distance escape outside of the bar-transgenic Zoysia japonica habitats were ascertained in terms of environmental factors affecting anthesis and pollen viability. In a 24-h day cycle, zoysiagrass pollen was released predominantly between 08:00 and 10:00, and the pollen was most viable during the same time interval. Optimal temperature and humidity for pollen viability was 15-20 °C and 80-90%, respectively. The pollen germinated in 120 min after anthesis, but under cloudy conditions germination time doubled. No differences in pollen viability/longevity between the transgenic and non-transgenic plants were observed. The pollen-mediated gene flow of transgenic Zoysia japonica to wild-type non-transgenic zoysiagrass species was monitored by measuring the cross-over rate of the bar gene in the context of three different models. At distances within 5 m, the rate of gene flow ranged from 3 to 5.7% according to the models used. The greater the distance from the transgenic plant site, the lower the gene flow rate. The furthest transgene detected was 38 m away and exhibited a 0.25% gene flow rate. The radial model yielded a 3.7% escape rate within a 3 m radius and was wind direction dependent. The distance- and direction-dependent gene flow events were influenced by wind direction and velocity during flowering season.
Keyword
TurfgrassZoysia japonicaHerbicide-resistantTransgenic zoysiagrassGene fow
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-021-00667-4
Type
Article
Appears in Collections:
National Research Safety Headquarter > 1. Journal Articles
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