DC Field | Value | Language |
---|---|---|
dc.contributor.author | C Wu | - |
dc.contributor.author | Y W Chen | - |
dc.contributor.author | M Scheible | - |
dc.contributor.author | C Chang | - |
dc.contributor.author | M Wittchen | - |
dc.contributor.author | Ju Huck Lee | - |
dc.contributor.author | T T Luong | - |
dc.contributor.author | B L Tiner | - |
dc.contributor.author | A Tauch | - |
dc.contributor.author | A Das | - |
dc.contributor.author | H Ton-That | - |
dc.date.accessioned | 2021-06-04T03:31:22Z | - |
dc.date.available | 2021-06-04T03:31:22Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/24376 | - |
dc.description.abstract | A gram-negative colonizer of the oral cavity, Fusobacterium nucleatum not only interacts with many pathogens in the oral microbiome but also has the ability to spread to extraoral sites including placenta and amniotic fluid, promoting preterm birth. To date, however, the molecular mechanism of interspecies interactions-termed coaggregation-by F. nucleatum and how coaggregation affects bacterial virulence remain poorly defined. Here, we employed genome-wide transposon mutagenesis to uncover fusobacterial coaggregation factors, revealing the intertwined function of a two-component signal transduction system (TCS), named CarRS, and a lysine metabolic pathway in regulating the critical coaggregation factor RadD. Transcriptome analysis shows that CarR modulates a large regulon including radD and lysine metabolic genes, such as kamA and kamD, the expression of which are highly up-regulated in the ΔcarR mutant. Significantly, the native culture medium of ΔkamA or ΔkamD mutants builds up abundant amounts of free lysine, which blocks fusobacterial coaggregation with streptococci. Our demonstration that lysine-conjugated beads trap RadD from the membrane lysates suggests that lysine utilizes RadD as its receptor to act as a metabolic inhibitor of coaggregation. Lastly, using a mouse model of preterm birth, we show that fusobacterial virulence is significantly attenuated with the ΔkamA and ΔcarR mutants, in contrast to the enhanced virulence phenotype observed upon diminishing RadD (ΔradD or ΔcarS mutant). Evidently, F. nucleatum employs the TCS CarRS and environmental lysine to modulate RadD-mediated interspecies interaction, virulence, and nutrient acquisition to thrive in the adverse environment of oral biofilms and extraoral sites. | - |
dc.publisher | Natl Acad Sciences | - |
dc.title | Genetic and molecular determinants of polymicrobial interactions in Fusobacterium nucleatum | - |
dc.title.alternative | Genetic and molecular determinants of polymicrobial interactions in Fusobacterium nucleatum | - |
dc.type | Article | - |
dc.citation.title | Proceedings of National Academy of Sciences of United States of America | - |
dc.citation.number | 23 | - |
dc.citation.endPage | 200648211 | - |
dc.citation.startPage | 200648211 | - |
dc.citation.volume | 118 | - |
dc.contributor.affiliatedAuthor | Ju Huck Lee | - |
dc.contributor.alternativeName | Wu | - |
dc.contributor.alternativeName | Chen | - |
dc.contributor.alternativeName | Scheible | - |
dc.contributor.alternativeName | 장천규 | - |
dc.contributor.alternativeName | Wittchen | - |
dc.contributor.alternativeName | 이주혁 | - |
dc.contributor.alternativeName | Luong | - |
dc.contributor.alternativeName | Tiner | - |
dc.contributor.alternativeName | Tauch | - |
dc.contributor.alternativeName | Das | - |
dc.contributor.alternativeName | Ton-That | - |
dc.identifier.bibliographicCitation | Proceedings of National Academy of Sciences of United States of America, vol. 118, no. 23, pp. 200648211-200648211 | - |
dc.identifier.doi | 10.1073/pnas.2006482118 | - |
dc.subject.keyword | Fusobacterium nucleatum | - |
dc.subject.keyword | Coaggregation | - |
dc.subject.keyword | Preterm birth | - |
dc.subject.keyword | Two-component transduction system | - |
dc.subject.keyword | Virulence | - |
dc.subject.local | Fusobacterium nucleatum | - |
dc.subject.local | Coaggregation | - |
dc.subject.local | preterm birth | - |
dc.subject.local | Preterm birth | - |
dc.subject.local | Preterm Birth | - |
dc.subject.local | Two-component transduction system | - |
dc.subject.local | virulence | - |
dc.subject.local | Virulence | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.