Exploration of alternative splicing events in mesenchymal stem cells from human induced pluripotent stem cells

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dc.contributor.authorJi Eun Jeong-
dc.contributor.authorBinna Seol-
dc.contributor.authorHan-Seop Kim-
dc.contributor.authorJae Yun Kim-
dc.contributor.authorYee Sook Cho-
dc.date.accessioned2021-06-04T03:31:47Z-
dc.date.available2021-06-04T03:31:47Z-
dc.date.issued2021-
dc.identifier.issn2073-4425-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24380-
dc.description.abstractAlthough comparative genome-wide transcriptomic analysis has provided insight into the biology of human induced pluripotent stem cell-derived mesenchymal stem cells (iMSCs), the distinct alternative splicing (AS) signatures of iMSCs remain elusive. Here, we performed Illumina RNA sequencing analysis to characterize AS events in iMSCs compared with tissue-derived MSCs. A total of 4586 differentially expressed genes (|FC| > 2) were identified between iMSCs and umbilical cord blood-derived MSCs (UCB-MSCs), including 2169 upregulated and 2417 downregulated genes. Of these, 164 differentially spliced events (BF > 20) in 112 genes were identified between iMSCs and UCB-MSCs. The predominant type of AS found in iMSCs was skipped exons (43.3%), followed by retained introns (19.5%), alternative 3′ (15.2%) and 5′ (12.8%) splice sites, and mutually exclusive exons (9.1%). Functional enrichment analysis showed that the differentially spliced genes (|FC| > 2 and BF > 20) were mainly enriched in functions associated with focal adhesion, extracellular exosomes, extracellular matrix organization, cell adhesion, and actin binding. Splice isoforms of selected genes including TRPT1, CNN2, and AP1G2, identified in sashimi plots, were further validated by RT-PCR analysis. This study provides valuable insight into the biology of iMSCs and the translation of mechanistic understanding of iMSCs into therapeutic applications.-
dc.publisherMDPI-
dc.titleExploration of alternative splicing events in mesenchymal stem cells from human induced pluripotent stem cells-
dc.title.alternativeExploration of alternative splicing events in mesenchymal stem cells from human induced pluripotent stem cells-
dc.typeArticle-
dc.citation.titleGenes-
dc.citation.number5-
dc.citation.endPage737-
dc.citation.startPage737-
dc.citation.volume12-
dc.contributor.affiliatedAuthorBinna Seol-
dc.contributor.affiliatedAuthorHan-Seop Kim-
dc.contributor.affiliatedAuthorJae Yun Kim-
dc.contributor.affiliatedAuthorYee Sook Cho-
dc.contributor.alternativeName정지은-
dc.contributor.alternativeName설빛나-
dc.contributor.alternativeName김한섭-
dc.contributor.alternativeName김재윤-
dc.contributor.alternativeName조이숙-
dc.identifier.bibliographicCitationGenes, vol. 12, no. 5, pp. 737-737-
dc.identifier.doi10.3390/genes12050737-
dc.subject.keywordHuman induced pluripotent stem cells-
dc.subject.keywordDifferentiation-
dc.subject.keywordMesenchymal stem cells-
dc.subject.keywordRNA sequencing-
dc.subject.keywordTranscriptome-
dc.subject.keywordAlternative splicing-
dc.subject.localHuman induced pluripotent stem cell-
dc.subject.localHuman induced pluripotent stem cells-
dc.subject.localDifferentiation-
dc.subject.localdifferentiation-
dc.subject.localmesenchymal stem cells-
dc.subject.localMesenchymal stem cell-
dc.subject.localMesenchymal stem cells-
dc.subject.localmesenchymal stem cells (MSCs)-
dc.subject.localRNA sequencing-
dc.subject.localrna sequence-
dc.subject.localRNA sequencing (RNA-seq)-
dc.subject.localTranscriptome-
dc.subject.localTranscriptomes-
dc.subject.localtranscriptome-
dc.subject.localAlternative splicing-
dc.subject.localalternative splicing-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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