An NIR dual-emitting/absorbing inorganic compact pair: a self-calibrating LRET system for homogeneous virus detection

Cited 0 time in scopus
Metadata Downloads
Title
An NIR dual-emitting/absorbing inorganic compact pair: a self-calibrating LRET system for homogeneous virus detection
Author(s)
D Kang; Hyun Joo Ahn; J Lee; S K Kim; J Pyun; C S Song; Sang Jick Kim; J Lee
Bibliographic Citation
Biosensors & Bioelectronics, vol. 190, pp. 113369-113369
Publication Year
2021
Abstract
Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.
Keyword
Lanthanide-doped nanoparticlesSingle-chain variable fragmentNear-infrared regionHomogeneous sandwich-immunoassayAvian origin-virus
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2021.113369
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.