An NIR dual-emitting/absorbing inorganic compact pair: a self-calibrating LRET system for homogeneous virus detection
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- An NIR dual-emitting/absorbing inorganic compact pair: a self-calibrating LRET system for homogeneous virus detection
- D Kang; Hyun Joo Ahn; J Lee; S K Kim; J Pyun; C S Song; Sang Jick Kim; J Lee
- Bibliographic Citation
- Biosensors & Bioelectronics, vol. 190, pp. 113369-113369
- Publication Year
- Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. The inorganic LRET pair was comprised of NIR dual-emitting lanthanide-doped nanoparticles (LnNPs) as donors and NIR-absorbing LnNPs as acceptors, which showed a narrow absorption peak (800 nm) and long-term stability, enabling stable LRET with a built-in self-calibrating signal. Screened single-chain variable fragments (scFvs) were used as target avian influenza virus (AIV)-binding antibodies to increase the LRET efficiency in sandwich-immunoassays. The compact sensor platform successfully detected AIV nucleoproteins with a 0.38 pM limit of detection in buffer solution and 64 clinical samples. Hence, inorganic LnNP pairs may be effective for self-calibrating LRET systems in the background-free NIR region.
- Lanthanide-doped nanoparticles; Single-chain variable fragment; Near-infrared region; Homogeneous sandwich-immunoassay; Avian origin-virus
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- 1. Journal Articles > Journal Articles
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