Production of porpyra-334 in transgenic lines of Nannochloropsis salina by the expression of mycosporine-like amino acid biosynthetic genes of P. yezoensis

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dc.contributor.authorJae-Sun In-
dc.contributor.authorJong Min Lim-
dc.contributor.authorSokyong Jung-
dc.contributor.authorD W Choi-
dc.contributor.authorSung Ran Min-
dc.contributor.authorWon Joong Jeong-
dc.date.accessioned2021-06-17T03:30:20Z-
dc.date.available2021-06-17T03:30:20Z-
dc.date.issued2021-
dc.identifier.issn0921-8971-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24402-
dc.description.abstractMycosporine-like amino acids (MAAs) are small secondary metabolites produced by some marine species. These compounds absorb ultraviolet (UV) light, typically between 310 and 362 nm, and protect the producers from UV-associated damage. They also have anti-photoaging, anticancer, and anti-inflammatory properties, which has generated considerable interest in the cosmetic, pharmaceutical, biotechnology, and materials fields. Commercial use has been limited due to its reliance marine organisms for production, resulting in low and inconsistent yields. This study was undertaken to increase production of MAAs. We generated transgenic lines of the Nannochloropsis salina that carry and express the MAA biosynthesis genes from red alga Pyropia yezoensis. We characterized their MAA yield and the properties of MAAs produced by these lines. When exposed to UV light, the transgenic lines generated fewer reactive oxygen species compared to wild-type. The yield of porphyra-334 in one of the transgenic lines was 25 mg g-1 dry cell weight, the greatest reported. We hope these results will support the use of MAAs in numerous applications and increase our understanding of their biosynthetic pathways.-
dc.publisherSpringer-
dc.titleProduction of porpyra-334 in transgenic lines of Nannochloropsis salina by the expression of mycosporine-like amino acid biosynthetic genes of P. yezoensis-
dc.title.alternativeProduction of porpyra-334 in transgenic lines of Nannochloropsis salina by the expression of mycosporine-like amino acid biosynthetic genes of P. yezoensis-
dc.typeArticle-
dc.citation.titleJournal of Applied Phycology-
dc.citation.number0-
dc.citation.endPage1672-
dc.citation.startPage1663-
dc.citation.volume33-
dc.contributor.affiliatedAuthorJae-Sun In-
dc.contributor.affiliatedAuthorJong Min Lim-
dc.contributor.affiliatedAuthorSokyong Jung-
dc.contributor.affiliatedAuthorSung Ran Min-
dc.contributor.affiliatedAuthorWon Joong Jeong-
dc.contributor.alternativeName인재선-
dc.contributor.alternativeName임종민-
dc.contributor.alternativeName정서경-
dc.contributor.alternativeName최동욱-
dc.contributor.alternativeName민성란-
dc.contributor.alternativeName정원중-
dc.identifier.bibliographicCitationJournal of Applied Phycology, vol. 33, pp. 1663-1672-
dc.identifier.doi10.1007/s10811-021-02396-1-
dc.subject.keywordMycosporine-like amino acids (MAAs)-
dc.subject.keywordPorphyra-334-
dc.subject.keywordNannochloropsis salina-
dc.subject.keywordPyropia yezoensis-
dc.subject.keywordHeterologous expression-
dc.subject.keywordProduction-
dc.subject.localMycosporine-like amino acids (MAAs)-
dc.subject.localPorphyra-334-
dc.subject.localNannochloropsis salina-
dc.subject.localPyropia yezoensis-
dc.subject.localheterologous expression-
dc.subject.localHeterologous expression-
dc.subject.localproduction-
dc.subject.localProduction-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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