A rapid quantitative on-site coronavirus disease 19 serological test

Cited 9 time in scopus
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Title
A rapid quantitative on-site coronavirus disease 19 serological test
Author(s)
J H Lee; P K Bae; H Kim; Y J Song; S Y Yi; J Kwon; J S Seo; J M Lee; H S Jo; S M Park; H S Park; K S Shin; S Chung; Yong Beom Shin
Bibliographic Citation
Biosensors & Bioelectronics, vol. 191, pp. 113406-113406
Publication Year
2021
Abstract
On-site severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) serological assays allow for timely in-field decisions to be made regarding patient status, also enabling population-wide screening to assist in controlling the coronavirus disease 2019 (COVID-19) pandemic. Here we propose a rapid microfluidic serological assay with two unique functions of nanointerstice filling and digitized flow control, which enable the fast/robust filling of the sample fluid as well as precise regulation of duration and volume of immune reaction. Developed microfluidic assay showed enhanced limit of detection, and 91.67% sensitivity and 100% specificity (n = 152) for clinical samples of SARS CoV-2 patients. The assay enables daily monitoring of IgM/IgG titers and patterns, which could be crucial parameters for convalescence from COVID-19 and provide important insight into how the immune system responds to SARS CoV-2. The developed on-site microfluidic assay presented the mean time for IgM and IgG seroconversions, indicating that these titers plateaued days after seroconversion. The mean duration from day 0 to PCR negativity was 19.4 days (median 20 d, IQR 16-21 d), with higher IgM/IgG titres being observed when PCR positive turns into negative. Simple monitoring of these titres promotes rapid on-site detection and comprehensive understanding of the immune response of COVID-19 patients.
Keyword
SARS-CoV-2COVID-19POCTMicrofluidicsImmunoassay
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2021.113406
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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