Sensitive detection of SARS-CoV-2 using a SERS-based aptasensor

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Title
Sensitive detection of SARS-CoV-2 using a SERS-based aptasensor
Author(s)
H Chen; S G Park; N Choi; Hyung-Jun KwonTaejoon Kang; M K Lee; J Choo
Bibliographic Citation
ACS Sensors, vol. 6, no. 6, pp. 2378-2385
Publication Year
2021
Abstract
We developed a new surface-enhanced Raman scattering (SERS)-based aptasensor platform capable of quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lysates with a high sensitivity. In this study, a spike protein deoxyribonucleic acid (DNA) aptamer was used as a receptor, and a self-grown Au nanopopcorn surface was used as a SERS detection substrate for the sensible detection of SARS-CoV-2. A quantitative analysis of the SARS-CoV-2 lysate was performed by monitoring the change in the SERS peak intensity caused by the new binding between the aptamer DNA released from the Au nanopopcorn surface and the spike protein in the SARS-CoV-2 virion. This technique enables detecting SARS-CoV-2 with a limit of detection (LoD) of less than 10 PFU/mL within 15 min. The results of this study demonstrate the possibility of a clinical application that can dramatically improve the detection limit and accuracy of the currently commercialized SARS-CoV-2 immunodiagnostic kit.
Keyword
SARS-CoV-2COVID-19Surface-enhanced Raman scatteringAptamerSpike protein
ISSN
2379-3694
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/acssensors.1c00596
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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