Luteolin orchestrates porcine oocyte meiotic progression by maintaining organelle dynamics under oxidative stress

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dc.contributor.authorSoo-Hyun Park-
dc.contributor.authorPil-Soo Jeong-
dc.contributor.authorYe Eun Joo-
dc.contributor.authorHyo-Gu Kang-
dc.contributor.authorMin Ju Kim-
dc.contributor.authorSanghoon Lee-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorS K Cho-
dc.contributor.authorBo Woong Sim-
dc.date.accessioned2021-07-06T03:31:03Z-
dc.date.available2021-07-06T03:31:03Z-
dc.date.issued2021-
dc.identifier.issn2296634X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24452-
dc.description.abstractIncreasing evidence has demonstrated that oxidative stress impairs oocyte maturation, but the underlying mechanisms remain largely unknown. Here, for the first time, we examined the antioxidant role of luteolin in meiotic progression and the underlying mechanisms. Supplementation of 5 μM luteolin increased the rates of first polar body extrusion and blastocyst formation after parthenogenetic activation, and the expression levels of oocyte competence (BMP15 and GDF9)-, mitogen-activated protein kinase (MOS)-, and maturation promoting factor (CDK1 and Cyclin B)-related genes were also improved. Luteolin supplementation decreased intracellular reactive oxygen species levels and increased the expression levels of oxidative stress-related genes (SOD1, SOD2, and CAT). Interestingly, luteolin alleviated defects in cell organelles, including actin filaments, the spindle, mitochondria, the endoplasmic reticulum, and cortical granules, caused by H2O2 exposure. Moreover, luteolin significantly improved the developmental competence of in vitro-fertilized embryos in terms of the cleavage rate, blastocyst formation rate, cell number, cellular survival rate, and gene expression and markedly restored the competencies decreased by H2O2 treatment. These findings revealed that luteolin supplementation during in vitro maturation improves porcine meiotic progression and subsequent embryonic development by protecting various organelle dynamics against oxidative stress, potentially increasing our understanding of the underlying mechanisms governing the relationship between oxidative stress and the meiotic events required for successful oocyte maturation.-
dc.publisherFrontiers Media Sa-
dc.titleLuteolin orchestrates porcine oocyte meiotic progression by maintaining organelle dynamics under oxidative stress-
dc.title.alternativeLuteolin orchestrates porcine oocyte meiotic progression by maintaining organelle dynamics under oxidative stress-
dc.typeArticle-
dc.citation.titleFrontiers in Cell and Developmental Biology-
dc.citation.number0-
dc.citation.endPage689826-
dc.citation.startPage689826-
dc.citation.volume9-
dc.contributor.affiliatedAuthorSoo-Hyun Park-
dc.contributor.affiliatedAuthorPil-Soo Jeong-
dc.contributor.affiliatedAuthorYe Eun Joo-
dc.contributor.affiliatedAuthorHyo-Gu Kang-
dc.contributor.affiliatedAuthorMin Ju Kim-
dc.contributor.affiliatedAuthorSanghoon Lee-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.contributor.alternativeName박수현-
dc.contributor.alternativeName정필수-
dc.contributor.alternativeName주예은-
dc.contributor.alternativeName강효구-
dc.contributor.alternativeName김민주-
dc.contributor.alternativeName이상훈-
dc.contributor.alternativeName송봉석-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName조성근-
dc.contributor.alternativeName심보웅-
dc.identifier.bibliographicCitationFrontiers in Cell and Developmental Biology, vol. 9, pp. 689826-689826-
dc.identifier.doi10.3389/fcell.2021.689826-
dc.subject.keywordLuteolin-
dc.subject.keywordAntioxidant-
dc.subject.keywordOxidative stress-
dc.subject.keywordOrganelle dynamics-
dc.subject.keywordIn vitro maturation-
dc.subject.keywordPorcine oocyte-
dc.subject.localLuteolin-
dc.subject.localAntioxidant-
dc.subject.localOxidative stress-
dc.subject.localIn vitro maturation-
dc.subject.localPorcine oocyte-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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