Detection of infectious white spot syndrome virus in red claw crayfish (Cherax quadricarinatus) and red swamp crayfish (Procambarus clarkii) imported into Korea

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Title
Detection of infectious white spot syndrome virus in red claw crayfish (Cherax quadricarinatus) and red swamp crayfish (Procambarus clarkii) imported into Korea
Author(s)
C Lee; Ji Hyung Kim; S K Choi; H J Jeon; S H Lee; B K Kim; Y K Kim; K J Lee; J E Han
Bibliographic Citation
Aquaculture, vol. 544, pp. 737117-737117
Publication Year
2021
Abstract
White spot syndrome virus (WSSV) is one of the most virulent viruses in crustaceans, causing severe economic losses to aquaculture industry. In this study, we tested for the presence of WSSV in the commodity crayfish imported to Korea and also examined the potential infectivity of WSSV in the commodity WSSV-positive crayfish. For the sampling, two species of crayfish, Cherax quadricarinatus (320 red claw crayfish in eight batches) imported from Indonesia and Procambarus clarkii (40 red swamp crayfish in two batches) imported from China, were obtained from fishery markets in Korea. Five representative samples per batch (N = 50) were screened for WSSV using PCR assay, and the virus was detected in five C. quadricarinatus samples and one P. clarkii sample. Among the WSSV-positive samples, the highest virus titer (the copies ranged between 4.93 × 105 and 8.36 × 106 copies/μL) was detected in the P. clarkii sample (20-012) by qPCR, and a comparatively low titer was observed in the C. quadricarinatus sample (20-008) (the copies ranged between 5.94 × 10 1 and 3.86 × 10 5 copies/μL). Following the virus infectivity test, WSSV infection was confirmed after feeding WSSV-positive crayfish tissue (20-008, 3.46 × 10 7 WSSV copies/μL) to Penaeus vannamei (Pacific white shrimp, N = 42) at 5% shrimp biomass. This is the first report of confirmed infectivity of WSSV from the commodity crayfish imported into Korea, suggesting that virus-infected frozen crayfish may present a potential source of viral disease in Korean shrimp aquaculture. Therefore, accurate and strict monitoring of crustacean viruses is required for imported crayfish from foreign countries into Korea.
Keyword
Crustacean virusesPenaeus vannameiBioassayAquacultureMonitoring
ISSN
0044-8486
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.aquaculture.2021.737117
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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