Syntrophic co-culture of a methanotroph and heterotroph for the efficient conversion of methane to mevalonate

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dc.contributor.authorHyewon Lee-
dc.contributor.authorJi In Baek-
dc.contributor.authorJin-Young Lee-
dc.contributor.authorJiyeong Jeong-
dc.contributor.authorHaseong Kim-
dc.contributor.authorDae Hee Lee-
dc.contributor.authorD M Kim-
dc.contributor.authorSeung Goo Lee-
dc.date.accessioned2021-07-26T15:30:34Z-
dc.date.available2021-07-26T15:30:34Z-
dc.date.issued2021-
dc.identifier.issn10967176-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24522-
dc.description.abstractAs the bioconversion of methane becomes increasingly important for bio-industrial and environmental applications, methanotrophs have received much attention for their ability to convert methane under ambient conditions. This includes the extensive reporting of methanotroph engineering for the conversion of methane to biochemicals. To further increase methane usability, we demonstrated a highly flexible and efficient modular approach based on a synthetic consortium of methanotrophs and heterotrophs mimicking the natural methane ecosystem to produce mevalonate (MVA) from methane. In the methane-conversion module, we used Methylococcus capsulatus Bath as a highly efficient methane biocatalyst and optimized the culture conditions for the production of high amounts of organic acids. In the MVA-synthesis module, we used Escherichia coli SBA01, an evolved strain with high organic acid tolerance and utilization ability, to convert organic acids to MVA. Using recombinant E. coli SBA01 possessing genes for the MVA pathway, 61 mg/L (0.4 mM) of MVA was successfully produced in 48 h without any addition of nutrients except methane. Our platform exhibited high stability and reproducibility with regard to cell growth and MVA production. We believe that this versatile system can be easily extended to many other value-added processes and has a variety of potential applications.-
dc.publisherElsevier-
dc.titleSyntrophic co-culture of a methanotroph and heterotroph for the efficient conversion of methane to mevalonate-
dc.title.alternativeSyntrophic co-culture of a methanotroph and heterotroph for the efficient conversion of methane to mevalonate-
dc.typeArticle-
dc.citation.titleMetabolic Engineering-
dc.citation.number0-
dc.citation.endPage292-
dc.citation.startPage285-
dc.citation.volume67-
dc.contributor.affiliatedAuthorHyewon Lee-
dc.contributor.affiliatedAuthorJi In Baek-
dc.contributor.affiliatedAuthorJin-Young Lee-
dc.contributor.affiliatedAuthorJiyeong Jeong-
dc.contributor.affiliatedAuthorHaseong Kim-
dc.contributor.affiliatedAuthorDae Hee Lee-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName이혜원-
dc.contributor.alternativeName백지인-
dc.contributor.alternativeName이진영-
dc.contributor.alternativeName정지영-
dc.contributor.alternativeName김하성-
dc.contributor.alternativeName이대희-
dc.contributor.alternativeName김동명-
dc.contributor.alternativeName이승구-
dc.identifier.bibliographicCitationMetabolic Engineering, vol. 67, pp. 285-292-
dc.identifier.doi10.1016/j.ymben.2021.07.008-
dc.subject.keywordCo-culture-
dc.subject.keywordMethane-
dc.subject.keywordMethanotroph-
dc.subject.keywordHeterotroph-
dc.subject.keywordMevalonate-
dc.subject.keywordBio-conversion-
dc.subject.localCo-culture-
dc.subject.localMethane-
dc.subject.localMevalonate-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Division of Biomaterials Research > 1. Journal Articles
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