TSA promotes CRISPR/Cas9 editing efficiency and expression of cell division-related genes from plant protoplasts

Cited 10 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSeung Hee Choi-
dc.contributor.authorM H Lee-
dc.contributor.authorDa Mon Jin-
dc.contributor.authorSu Ji Ju-
dc.contributor.authorWoo-Seok Ahn-
dc.contributor.authorEun Yee Jie-
dc.contributor.authorJi Min Lee-
dc.contributor.authorJiyoung Lee-
dc.contributor.authorCha Young Kim-
dc.contributor.authorSuk Weon Kim-
dc.date.accessioned2021-08-03T15:30:30Z-
dc.date.available2021-08-03T15:30:30Z-
dc.date.issued2021-
dc.identifier.issn1661-6596-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24589-
dc.description.abstractTrichostatin A (TSA) is a representative histone deacetylase (HDAC) inhibitor that modulates epigenetic gene expression by regulation of chromatin remodeling in cells. To investigate whether the regulation of chromatin de-condensation by TSA can affect the increase in the efficiency of Cas9 protein-gRNA ribonucleoprotein (RNP) indel formation from plant cells, genome editing efficiency using lettuce and tobacco protoplasts was examined after several concentrations of TSA treatments (0, 0.1, 1 and 10 μM). RNP delivery from protoplasts was conducted by conventional polyethylene glycol (PEG) transfection protocols. Interestingly, the indel frequency of the SOC1 gene from TSA treatments was about 3.3 to 3.8 times higher than DMSO treatment in lettuce protoplasts. The TSA-mediated increase of indel frequency of the SOC1 gene in lettuce protoplasts occurred in a concentration-dependent manner, although there was not much difference. Similar to lettuce, TSA also increased the indel frequency by 1.5 to 1.8 times in a concentration-dependent manner during PDS genome editing using tobacco protoplasts. The MNase test clearly showed that chromatin accessibility with TSA treatments was higher than that of DMSO treatment. Additionally, TSA treatment significantly increased the level of histone H3 and H4 acetylation from lettuce protoplasts. The qRT-PCR analysis showed that expression of cell division-related genes (LsCYCD1-1, LsCYCD3-2, LsCYCD6-1, and LsCYCU4-1) was increased by TSA treatment. These findings could contribute to increasing the efficiency of CRISPR/Cas9-mediated genome editing. Furthermore, this could be applied for the development of useful genome-edited crops using the CRISPR/Cas9 system with plant protoplasts. ⓒ 2021 by the authors. Licensee MDPI, Basel, Switzerland.-
dc.publisherMDPI-
dc.titleTSA promotes CRISPR/Cas9 editing efficiency and expression of cell division-related genes from plant protoplasts-
dc.title.alternativeTSA promotes CRISPR/Cas9 editing efficiency and expression of cell division-related genes from plant protoplasts-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Sciences-
dc.citation.number15-
dc.citation.endPage7817-
dc.citation.startPage7817-
dc.citation.volume22-
dc.contributor.affiliatedAuthorSeung Hee Choi-
dc.contributor.affiliatedAuthorDa Mon Jin-
dc.contributor.affiliatedAuthorSu Ji Ju-
dc.contributor.affiliatedAuthorWoo-Seok Ahn-
dc.contributor.affiliatedAuthorEun Yee Jie-
dc.contributor.affiliatedAuthorJi Min Lee-
dc.contributor.affiliatedAuthorJiyoung Lee-
dc.contributor.affiliatedAuthorCha Young Kim-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.alternativeName최승희-
dc.contributor.alternativeName이명희-
dc.contributor.alternativeName진다몬-
dc.contributor.alternativeName주수지-
dc.contributor.alternativeName안우석-
dc.contributor.alternativeName지은이-
dc.contributor.alternativeName이지민-
dc.contributor.alternativeName이지영-
dc.contributor.alternativeName김차영-
dc.contributor.alternativeName김석원-
dc.identifier.bibliographicCitationInternational Journal of Molecular Sciences, vol. 22, no. 15, pp. 7817-7817-
dc.identifier.doi10.3390/ijms22157817-
dc.subject.keywordTrichostatin A-
dc.subject.keywordGenome editing efficiency-
dc.subject.keywordHistone acetylation-
dc.subject.keywordChromatin de-condensation-
dc.subject.keywordPlant protoplasts-
dc.subject.keywordLettuce-
dc.subject.keywordTobacco-
dc.subject.localTrichostatin A-
dc.subject.localtrichostatin A-
dc.subject.localTrichostatin A (TSA)-
dc.subject.localGenome editing efficiency-
dc.subject.localHistone acetylation-
dc.subject.localhistone acetylatione-
dc.subject.localhistone acetylation-
dc.subject.localChromatin de-condensation-
dc.subject.localPlant protoplasts-
dc.subject.localLettuce-
dc.subject.localNicotiana tabacum-
dc.subject.localNicotiana tabacum L.-
dc.subject.localNicotiana tabacum tobacco-
dc.subject.localTobacco-
dc.subject.localTobacco (Nicotiana tabacum L.)-
dc.subject.localnicotiana tabacum-
dc.subject.localtobacco-
dc.subject.localtobacco (nicotiana tabacum)-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.