(-)-α-bisabolol production in engineered Escherichia coli expressing a novel (-)-α-bisabolol synthase from the globe artichoke Cynara cardunculus var. Scolymus

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dc.contributor.authorHyun Seung Lim-
dc.contributor.authorSeong Keun Kim-
dc.contributor.authorSeung Gyun Woo-
dc.contributor.authorTae Hyun Kim-
dc.contributor.authorS J Yeom-
dc.contributor.authorWonshik Yong-
dc.contributor.authorY J Ko-
dc.contributor.authorSoo Jung Kim-
dc.contributor.authorSeung Goo Lee-
dc.contributor.authorDae Hee Lee-
dc.date.accessioned2021-08-09T15:30:49Z-
dc.date.available2021-08-09T15:30:49Z-
dc.date.issued2021-
dc.identifier.issn00218561-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24624-
dc.description.abstract(-)-α-Bisabolol is a functional ingredient in various health and cosmetic products and has antibacterial, anti-inflammatory, and wound healing properties. (-)-α-Bisabolol is chemically synthesized and produced by steam distillation of essential oils extracted from Brazilian Candeia (Eremanthus erythropappus). To sustainably produce pure (-)-α-bisabolol, we previously engineered Escherichia coli to produce 9.1 g/L (-)-α-bisabolol via heterologous mevalonate pathways and (-)-α-bisabolol synthase (BOS) from German chamomile, Matricaria recutita (MrBOS). BOS has only been reported in MrBOS and Brazilian Candeia (EeBOS). The limited availability of BOS has made it difficult to achieve high titer and yield and large-scale (-)-α-bisabolol production. We identified a novel BOS in globe artichoke (CcBOS) and examined its functionality in vitro and in vivo. CcBOS showed higher catalytic efficiency and (-)-α-bisabolol production rates than those from MrBOS or EeBOS. In fed-batch fermentation, CcBOS generated the highest reported (-)-α-bisabolol titer to date (23.4 g/L). These results may facilitate economically viable industrial (-)-α-bisabolol production.-
dc.publisherAmer Chem Soc-
dc.title(-)-α-bisabolol production in engineered Escherichia coli expressing a novel (-)-α-bisabolol synthase from the globe artichoke Cynara cardunculus var. Scolymus-
dc.title.alternative(-)-α-bisabolol production in engineered Escherichia coli expressing a novel (-)-α-bisabolol synthase from the globe artichoke Cynara cardunculus var. Scolymus-
dc.typeArticle-
dc.citation.titleJournal of Agricultural and Food Chemistry-
dc.citation.number30-
dc.citation.endPage8503-
dc.citation.startPage8492-
dc.citation.volume69-
dc.contributor.affiliatedAuthorHyun Seung Lim-
dc.contributor.affiliatedAuthorSeong Keun Kim-
dc.contributor.affiliatedAuthorSeung Gyun Woo-
dc.contributor.affiliatedAuthorTae Hyun Kim-
dc.contributor.affiliatedAuthorWonshik Yong-
dc.contributor.affiliatedAuthorSoo Jung Kim-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.affiliatedAuthorDae Hee Lee-
dc.contributor.alternativeName임현승-
dc.contributor.alternativeName김성근-
dc.contributor.alternativeName우승균-
dc.contributor.alternativeName김태현-
dc.contributor.alternativeName염수진-
dc.contributor.alternativeName용원식-
dc.contributor.alternativeName고윤주-
dc.contributor.alternativeName김수정-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName이대희-
dc.identifier.bibliographicCitationJournal of Agricultural and Food Chemistry, vol. 69, no. 30, pp. 8492-8503-
dc.identifier.doi10.1021/acs.jafc.1c02759-
dc.subject.keyword(-)-α-bisabolol-
dc.subject.keyword(-)-α-bisabolol synthase-
dc.subject.keywordEscherichia coli-
dc.subject.keywordFermentation-
dc.subject.keywordMevalonate pathway-
dc.subject.keywordArtichoke-
dc.subject.localEscherichia coli-
dc.subject.localFermentation-
dc.subject.localMevalonate pathway-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Division of Biomaterials Research > 1. Journal Articles
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