Multivalent sialyllactose-levan-conjugated gold nanoparticles for efficient interaction with and colorimetric detection of influenza A virus

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Title
Multivalent sialyllactose-levan-conjugated gold nanoparticles for efficient interaction with and colorimetric detection of influenza A virus
Author(s)
S J Kim; P K Bae; Yong Beom Shin
Bibliographic Citation
Chemosensors, vol. 9, no. 7, pp. 186-186
Publication Year
2021
Abstract
We report a colorimetric assay to detect influenza A virus using sialyllactose-levan-conju-gated gold nanoparticles (AuNPs). We successfully conjugated 2, 3-and 2, 6-sialyllactose to levan and synthesized sialyllactose-levan-conjugated AuNPs. Each sialyllactose-conjugated levan specifically interacted with a recognizable lectin. Synthesized sialyllactose-conjugated levan acted as re-ducing and coating agents during the formation of AuNPs. Human influenza A virus specifically bound to 2, 6-sialyllactose-levan-conjugated AuNPs. Moreover, 2, 6-sialyllactose-conjugated levan AuNPs rapidly changed color from red to blue after incubation with human influenza virus. For detecting avian influenza virus, 2, 3-sialyllactose-levan-conjugated AuNPs were more effective than 2, 6-sialyllactose-levan-conjugated AuNPs. Therefore, the efficient targeting and diagnosis of influenza virus according to origin was possible. The deployment of sialyllactose-levan-conjugated par-ticles for the detection of influenza virus is simple and quick. The limit of detection (L.O.D) of H1N1 influenza virus was 7.4 × 103 pfu using 2, 6-siallylactose-levan-conjugated AuNPs and H5N2 influenza virus was 4.2 × 103 pfu using 2, 3-siallylactose-levan-conjugated AuNPs. ⓒ 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keyword
LevanSialyllactoseGold nanoparticleColorimetric assayInfluenza
ISSN
2227-9240
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/chemosensors9070186
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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