Overexpression of 4-hydroxyphenylpyruvate dioxygenase (IbHPPD) increases abiotic stress tolerance in transgenic sweetpotato plants

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dc.contributor.authorSo-Eun Kim-
dc.contributor.authorX Bian-
dc.contributor.authorChan-Ju Lee-
dc.contributor.authorSul-U Park-
dc.contributor.authorYehoon Leem-
dc.contributor.authorBeg Hab Kim-
dc.contributor.authorW S Park-
dc.contributor.authorM J Ahn-
dc.contributor.authorC Y Ji-
dc.contributor.authorY Yu-
dc.contributor.authorY Xie-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorHo Soo Kim-
dc.date.accessioned2021-08-23T15:30:20Z-
dc.date.available2021-08-23T15:30:20Z-
dc.date.issued2021-
dc.identifier.issn0981-9428-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24660-
dc.description.abstractTocopherols are lipid-soluble compounds regarded as vitamin E compounds and they function as antioxidants in scavenging lipid peroxyl radicals and quenching reactive oxygen species (ROS). In our previous studies, we isolated five tocopherol biosynthesis genes from sweetpotato (Ipomoea batatas [L.] Lam) plants including 4-hydroxyphenylpyruvate dioxygenase (IbHPPD). HPPD is the first regulatory enzyme in vitamin E biosynthesis and serves to catalyze in the first steps α-tocopherol and plastoquinone biosynthesis by converting 4-hydroxyphenylpyruvate (HPP) to homogentisic acid (HGA). In this study, we generated transgenic sweetpotato plants overexpressing IbHPPD under the control of cauliflower mosaic virus (CaMV) 35S promoter (referred to as HP plants) via Agrobacterium-mediated transformation to understand the function of IbHPPD in sweetpotato. Three transgenic lines (HP3, HP14 and HP15) with high transcript levels of IbHPPD were selected for further characterization. Compared with non-transgenic (NT) plants, HP plants exhibited enhanced tolerance to multiple environmental stresses, including salt, drought, and oxidative stresses. In addition, HP plants showed increased tolerance to the herbicide sulcotrione, which is involved in the inhibition of the HPPD. Interestingly, after stress treatments, HP plants also showed higher abscisic acid (ABA) contents than NT plants. Under dehydrated condition, HP plants displayed an elevated α-tocopherol content to 19-27% in leaves compared with NT plants. These results indicate that increased abiotic stress tolerance in HP plants is related to inducing enhancement of α-tocopherol and ABA contents.-
dc.publisherElsevier-
dc.titleOverexpression of 4-hydroxyphenylpyruvate dioxygenase (IbHPPD) increases abiotic stress tolerance in transgenic sweetpotato plants-
dc.title.alternativeOverexpression of 4-hydroxyphenylpyruvate dioxygenase (IbHPPD) increases abiotic stress tolerance in transgenic sweetpotato plants-
dc.typeArticle-
dc.citation.titlePlant Physiology and Biochemistry-
dc.citation.number0-
dc.citation.endPage429-
dc.citation.startPage420-
dc.citation.volume167-
dc.contributor.affiliatedAuthorSo-Eun Kim-
dc.contributor.affiliatedAuthorChan-Ju Lee-
dc.contributor.affiliatedAuthorSul-U Park-
dc.contributor.affiliatedAuthorYehoon Leem-
dc.contributor.affiliatedAuthorBeg Hab Kim-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.affiliatedAuthorHo Soo Kim-
dc.contributor.alternativeName김소은-
dc.contributor.alternativeNameBian-
dc.contributor.alternativeName이찬주-
dc.contributor.alternativeName박슬우-
dc.contributor.alternativeName임예훈-
dc.contributor.alternativeName김백합-
dc.contributor.alternativeName박우성-
dc.contributor.alternativeName안미정-
dc.contributor.alternativeName지창윤-
dc.contributor.alternativeNameYu-
dc.contributor.alternativeNameXie-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeName김호수-
dc.identifier.bibliographicCitationPlant Physiology and Biochemistry, vol. 167, pp. 420-429-
dc.identifier.doi10.1016/j.plaphy.2021.08.025-
dc.subject.keywordSweetpotato-
dc.subject.keywordIbHPPD-
dc.subject.keywordTocopherol-
dc.subject.keywordHerbicide stress-
dc.subject.keywordAbiotic stress-
dc.subject.localsweet potato-
dc.subject.localsweet potatoes-
dc.subject.localSweet potato (Ipomoea batatas L. Lam)-
dc.subject.localSweet potato (Ipomoea batatas)-
dc.subject.localSweet otato-
dc.subject.localsweet potato (Ipomoea batatas)-
dc.subject.localipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas)-
dc.subject.localsweetpotato-
dc.subject.localSweetpotato Ipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas L.)-
dc.subject.localSweetpotato-
dc.subject.localSweetpotato (Ipomoea batatas (L.) Lam)-
dc.subject.localSweet potato-
dc.subject.localIpomoea batatas-
dc.subject.localSweet potato (Ipomoea batatas (L.) Lam.)-
dc.subject.localIbHPPD-
dc.subject.localtocopherols-
dc.subject.localTocopherols-
dc.subject.localTocopherol-
dc.subject.localHerbicide stress-
dc.subject.localAbiotic stresses-
dc.subject.localAbiotic stress-
dc.subject.localabiotic stress-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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