Immunomagnetic microfluidic integrated system for potency-based multiple separation of heterogeneous stem cells with high throughput capabilities = 미세유체칩을 이용한 iPSCs 분리 시스템 개발

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Title
Immunomagnetic microfluidic integrated system for potency-based multiple separation of heterogeneous stem cells with high throughput capabilities = 미세유체칩을 이용한 iPSCs 분리 시스템 개발
Author(s)
Byunghoon Kang; S Han; H Y Son; B Mun; M K Shin; Y Choi; Jongjin Park; Jeong Ki Min; D Park; Eun Kyung Lim; Y M Huh; S Haam
Bibliographic Citation
Biosensors & Bioelectronics, vol. 194, pp. 113576-113576
Publication Year
2021
Abstract
Multipotent adult stem cells (MASCs) derived from Pluripotent stem cells (PSCs) have found widespread use in various applications, including regenerative therapy and drug screening. For these applications, highly pluripotent PSCs need to be selectively separated from those that show low pluripotency for reusage of PSCs, and MASCs need to be collected for further application. Herein, we developed immunomagnetic microfluidic integrated system (IM-MIS) for separation of stem cells depending on potency level. In this system, each stem cell was multiple-separated in microfluidics chip by magnetophoretic mobility of magnetic-activated cells based on the combination of two sizes of magnetic nanoparticles and two different antibodies. Magnetic particles had a difference in the degree of magnetization, and antibodies recognized potency-related surface markers. IM-MIS showed superior cell separation performance than FACS with high throughput (49.5%) in a short time (<15 min) isolate 1 × 107 cells, and higher purity (92.1%) than MACS. IM-MIS had a cell viability of 89.1%, suggesting that IM-MIS had no effect on cell viability during isolation. Furthermore, IM-MIS did not affect the key characteristics of stem cells including its differentiation potency, phenotype, genotype, and karyotype. IM-MIS may offer a new platform for the development of multi-separation systems for diverse stem cell applications.
Keyword
Magnetic nanoparticleMagnetophoresisMicrofluidic systemInduced pluripotent stem cellBone-marrow mesenchymal stem cell
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2021.113576
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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