Development of antibody against drug-resistant respiratory syncytial virus: rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHyeran Kim-
dc.contributor.authorSeul Gee Hwang-
dc.contributor.authorKyeonghye Guk-
dc.contributor.authorYoonji Bae-
dc.contributor.authorH Park-
dc.contributor.authorEun Kyung Lim-
dc.contributor.authorTaejoon Kang-
dc.contributor.authorJuyeon Jung-
dc.date.accessioned2021-09-06T15:30:13Z-
dc.date.available2021-09-06T15:30:13Z-
dc.date.issued2021-
dc.identifier.issn09565663-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/24699-
dc.description.abstractRespiratory syncytial virus (RSV) infections are associated with severe bronchiolitis or pneumonia. Although palivizumab is used to prevent RSV infections, the occurrence of palivizumab-resistant RSV strains is increasing, and these strains pose a threat to public health. Herein, we report an antibody with affinity to the S275F RSV antigen, enabling the specific detection of palivizumab-resistant RSV strains. Experimental and simulation results confirmed the affinity of the antibody to the S275F RSV antigen. Furthermore, we developed a rapid S275F RSV antigen detection method using a split superfolder green fluorescent protein (ssGFP) that can interact with the antibody. In the presence of the mutant virus antigen, ssGFP emitted fluorescence within 1 min, allowing the rapid identification of S275F RSV. We anticipate that the developed antibody would be useful for the precise diagnosis of antiviral drug-resistant RSV strains and help treat patients with RSV infections.-
dc.publisherElsevier-
dc.titleDevelopment of antibody against drug-resistant respiratory syncytial virus: rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system-
dc.title.alternativeDevelopment of antibody against drug-resistant respiratory syncytial virus: rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number0-
dc.citation.endPage113593-
dc.citation.startPage113593-
dc.citation.volume194-
dc.contributor.affiliatedAuthorHyeran Kim-
dc.contributor.affiliatedAuthorSeul Gee Hwang-
dc.contributor.affiliatedAuthorKyeonghye Guk-
dc.contributor.affiliatedAuthorYoonji Bae-
dc.contributor.affiliatedAuthorEun Kyung Lim-
dc.contributor.affiliatedAuthorTaejoon Kang-
dc.contributor.affiliatedAuthorJuyeon Jung-
dc.contributor.alternativeName김혜란-
dc.contributor.alternativeName황슬기-
dc.contributor.alternativeName국경혜-
dc.contributor.alternativeName배윤지-
dc.contributor.alternativeName박황서-
dc.contributor.alternativeName임은경-
dc.contributor.alternativeName강태준-
dc.contributor.alternativeName정주연-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 194, pp. 113593-113593-
dc.identifier.doi10.1016/j.bios.2021.113593-
dc.subject.keywordAntibody-
dc.subject.keywordDrug-resistance-
dc.subject.keywordS275F mutation-
dc.subject.keywordRespiratory syncytial virus-
dc.subject.keywordGreen fluorescent protein-
dc.subject.localAntibody-
dc.subject.localDrug-resistance-
dc.subject.localGreen fluorescent protein-
dc.description.journalClassY-
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.