Cited 3 time in
- Title
- Attenuation of experimental autoimmune uveitis in Lewis rats by betaine
- Author(s)
- Y Choi; Kyungsook Jung; H J Kim; J Chun; M Ahn; Y Jee; H J Ko; C Moon; H Matsuda; A Tanaka; J Kim; T Shin
- Bibliographic Citation
- Experimental Neurobiology, vol. 30, no. 4, pp. 308-317
- Publication Year
- 2021
- Abstract
- Experimental autoimmune uveitis (EAU) is an animal model of human autoimmune uveitis that is characterized by the infiltration of autoimmune T cells with concurrent increases in pro-inflammatory cytokines and reactive oxygen species. This study aimed to assess whether betaine regulates the progression of EAU in Lewis rats. EAU was induced via immunization with the interphotoreceptor retinoid-binding protein (IRBP) and oral administration of either a vehicle or betaine (100 mg/kg) for 9 consecutive days. Spleens, blood, and retinas were sampled from the experimental rats at the time of sacrifice and used for the T cell proliferation assay, serological analysis, real-time polymerase chain reaction, and immunohistochemistry. The T cell proliferation assay revealed that betaine had little effect on the proliferation of splenic T cells against the IRBP antigen in an in vitro assay on day 9 post-immunization. The serological analysis showed that the level of serum superoxide dismutase increased in the betainetreated group compared with that in the vehicle-treated group. The anti-inflammatory effect of betaine was confirmed by the downregulation of pro-inflammation-related molecules, including vascular cell adhesion molecule 1 and interleukin-1β in the retinas of rats with EAU. The histopathological findings agreed with those of ionized calcium-binding adaptor molecule 1 immunohistochemistry, further verifying that inflammation in the retina and ciliary bodies was significantly suppressed in the betaine-treated group compared with the vehicle-treated group. Results of the present study suggest that betaine is involved in mitigating EAU through anti-oxidation and anti-inflammatory activities.
- Keyword
- Anti-inflammationAnti-oxidationBetaineExperimental autoimmune uveitisRetina
- ISSN
- 1226-2560
- Publisher
- Korea Soc-Assoc-Inst
- DOI
- http://dx.doi.org/10.5607/en21011
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
- Files in This Item:
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