Combined chaetocin/trichostatin A treatment improves the epigenetic modification and developmental competence of porcine somatic cell nuclear transfer embryos

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dc.contributor.authorPil-Soo Jeong-
dc.contributor.authorHae Jun Yang-
dc.contributor.authorSoo-Hyun Park-
dc.contributor.authorMin Ah Gwon-
dc.contributor.authorYe Eun Joo-
dc.contributor.authorMin Ju Kim-
dc.contributor.authorHyo-Gu Kang-
dc.contributor.authorSanghoon Lee-
dc.contributor.authorYoung-Ho Park-
dc.contributor.authorBong-Seok Song-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorD B Koo-
dc.contributor.authorBo Woong Sim-
dc.description.abstractDevelopmental defects in somatic cell nuclear transfer (SCNT) embryos are principally attributable to incomplete epigenetic reprogramming. Small-molecule inhibitors such as histone methyltransferase inhibitors (HMTi) and histone deacetylase inhibitors (HDACi) have been used to improve reprogramming efficiency of SCNT embryos. However, their possible synergistic effect on epigenetic reprogramming has not been studied. In this study, we explored whether combined treatment with an HMTi (chaetocin) and an HDACi (trichostatin A; TSA) synergistically enhanced epigenetic reprogramming and the developmental competence of porcine SCNT embryos. Chaetocin, TSA, and the combination significantly increased the cleavage and blastocyst formation rate, hatching/hatched blastocyst rate, and cell numbers and survival rate compared to control embryos. In particular, the combined treatment improved the rate of development to blastocysts more so than chaetocin or TSA alone. TSA and combined chaetocin/TSA significantly reduced the H3K9me3 levels and increased the H3K9ac levels in SCNT embryos, although chaetocin alone significantly reduced only the H3K9me3 levels. Moreover, these inhibitors also decreased global DNA methylation in SCNT embryos. In addition, the expression of zygotic genome activation- and imprinting-related genes was increased by chaetocin or TSA, and more so by the combination, to levels similar to those of in vitro-fertilized embryos. These results suggest that combined chaetocin/TSA have synergistic effects on improving the developmental competences by regulating epigenetic reprogramming and correcting developmental potential-related gene expression in porcine SCNT embryos. Therefore, these strategies may contribute to the generation of transgenic pigs for biomedical research.-
dc.publisherFrontiers Media Sa-
dc.titleCombined chaetocin/trichostatin A treatment improves the epigenetic modification and developmental competence of porcine somatic cell nuclear transfer embryos-
dc.title.alternativeCombined chaetocin/trichostatin A treatment improves the epigenetic modification and developmental competence of porcine somatic cell nuclear transfer embryos-
dc.citation.titleFrontiers in Cell and Developmental Biology-
dc.contributor.affiliatedAuthorPil-Soo Jeong-
dc.contributor.affiliatedAuthorHae Jun Yang-
dc.contributor.affiliatedAuthorSoo-Hyun Park-
dc.contributor.affiliatedAuthorMin Ah Gwon-
dc.contributor.affiliatedAuthorYe Eun Joo-
dc.contributor.affiliatedAuthorMin Ju Kim-
dc.contributor.affiliatedAuthorHyo-Gu Kang-
dc.contributor.affiliatedAuthorSanghoon Lee-
dc.contributor.affiliatedAuthorYoung-Ho Park-
dc.contributor.affiliatedAuthorBong-Seok Song-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorBo Woong Sim-
dc.identifier.bibliographicCitationFrontiers in Cell and Developmental Biology, vol. 9, pp. 709574-709574-
dc.subject.keywordTrichostatin A (TSA)-
dc.subject.keywordSomatic cell nuclear transfer (SCNT)-
dc.subject.keywordPorcine embryonic development-
dc.subject.keywordEpigenetic reprogramming-
dc.subject.keywordZygotic genome activation-
dc.subject.keywordGenomic imprinting-
dc.subject.localSomatic cell nuclear transfer (SCNT)-
dc.subject.localSomatic cell nuclear transfer-
dc.subject.localEpigenetic reprogramming-
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Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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