DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pil-Soo Jeong | - |
dc.contributor.author | Hae Jun Yang | - |
dc.contributor.author | Soo-Hyun Park | - |
dc.contributor.author | Min Ah Gwon | - |
dc.contributor.author | Ye Eun Joo | - |
dc.contributor.author | Min Ju Kim | - |
dc.contributor.author | Hyo-Gu Kang | - |
dc.contributor.author | Sanghoon Lee | - |
dc.contributor.author | Young-Ho Park | - |
dc.contributor.author | Bong-Seok Song | - |
dc.contributor.author | Sun-Uk Kim | - |
dc.contributor.author | D B Koo | - |
dc.contributor.author | Bo Woong Sim | - |
dc.date.accessioned | 2021-10-26T15:30:45Z | - |
dc.date.available | 2021-10-26T15:30:45Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 2296-634X | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/24945 | - |
dc.description.abstract | Developmental defects in somatic cell nuclear transfer (SCNT) embryos are principally attributable to incomplete epigenetic reprogramming. Small-molecule inhibitors such as histone methyltransferase inhibitors (HMTi) and histone deacetylase inhibitors (HDACi) have been used to improve reprogramming efficiency of SCNT embryos. However, their possible synergistic effect on epigenetic reprogramming has not been studied. In this study, we explored whether combined treatment with an HMTi (chaetocin) and an HDACi (trichostatin A; TSA) synergistically enhanced epigenetic reprogramming and the developmental competence of porcine SCNT embryos. Chaetocin, TSA, and the combination significantly increased the cleavage and blastocyst formation rate, hatching/hatched blastocyst rate, and cell numbers and survival rate compared to control embryos. In particular, the combined treatment improved the rate of development to blastocysts more so than chaetocin or TSA alone. TSA and combined chaetocin/TSA significantly reduced the H3K9me3 levels and increased the H3K9ac levels in SCNT embryos, although chaetocin alone significantly reduced only the H3K9me3 levels. Moreover, these inhibitors also decreased global DNA methylation in SCNT embryos. In addition, the expression of zygotic genome activation- and imprinting-related genes was increased by chaetocin or TSA, and more so by the combination, to levels similar to those of in vitro-fertilized embryos. These results suggest that combined chaetocin/TSA have synergistic effects on improving the developmental competences by regulating epigenetic reprogramming and correcting developmental potential-related gene expression in porcine SCNT embryos. Therefore, these strategies may contribute to the generation of transgenic pigs for biomedical research. | - |
dc.publisher | Frontiers Media Sa | - |
dc.title | Combined chaetocin/trichostatin A treatment improves the epigenetic modification and developmental competence of porcine somatic cell nuclear transfer embryos | - |
dc.title.alternative | Combined chaetocin/trichostatin A treatment improves the epigenetic modification and developmental competence of porcine somatic cell nuclear transfer embryos | - |
dc.type | Article | - |
dc.citation.title | Frontiers in Cell and Developmental Biology | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 709574 | - |
dc.citation.startPage | 709574 | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Pil-Soo Jeong | - |
dc.contributor.affiliatedAuthor | Hae Jun Yang | - |
dc.contributor.affiliatedAuthor | Soo-Hyun Park | - |
dc.contributor.affiliatedAuthor | Min Ah Gwon | - |
dc.contributor.affiliatedAuthor | Ye Eun Joo | - |
dc.contributor.affiliatedAuthor | Min Ju Kim | - |
dc.contributor.affiliatedAuthor | Hyo-Gu Kang | - |
dc.contributor.affiliatedAuthor | Sanghoon Lee | - |
dc.contributor.affiliatedAuthor | Young-Ho Park | - |
dc.contributor.affiliatedAuthor | Bong-Seok Song | - |
dc.contributor.affiliatedAuthor | Sun-Uk Kim | - |
dc.contributor.affiliatedAuthor | Bo Woong Sim | - |
dc.contributor.alternativeName | 정필수 | - |
dc.contributor.alternativeName | 양해준 | - |
dc.contributor.alternativeName | 박수현 | - |
dc.contributor.alternativeName | 권민아 | - |
dc.contributor.alternativeName | 주예은 | - |
dc.contributor.alternativeName | 김민주 | - |
dc.contributor.alternativeName | 강효구 | - |
dc.contributor.alternativeName | 이상훈 | - |
dc.contributor.alternativeName | 박영호 | - |
dc.contributor.alternativeName | 송봉석 | - |
dc.contributor.alternativeName | 김선욱 | - |
dc.contributor.alternativeName | 구덕본 | - |
dc.contributor.alternativeName | 심보웅 | - |
dc.identifier.bibliographicCitation | Frontiers in Cell and Developmental Biology, vol. 9, pp. 709574-709574 | - |
dc.identifier.doi | 10.3389/fcell.2021.709574 | - |
dc.subject.keyword | Chaetocin | - |
dc.subject.keyword | Trichostatin A (TSA) | - |
dc.subject.keyword | Somatic cell nuclear transfer (SCNT) | - |
dc.subject.keyword | Porcine embryonic development | - |
dc.subject.keyword | Epigenetic reprogramming | - |
dc.subject.keyword | Zygotic genome activation | - |
dc.subject.keyword | Genomic imprinting | - |
dc.subject.local | chaetocin | - |
dc.subject.local | Chaetocin | - |
dc.subject.local | trichostatin A | - |
dc.subject.local | Trichostatin A (TSA) | - |
dc.subject.local | Trichostatin A | - |
dc.subject.local | somatic cell nuclear transfer | - |
dc.subject.local | Somatic cell nuclear transfer | - |
dc.subject.local | Somatic cell nuclear transfer (SCNT) | - |
dc.subject.local | Porcine embryonic development | - |
dc.subject.local | Epigenetic reprogramming | - |
dc.subject.local | epigenetic reprogramming | - |
dc.subject.local | Zygotic genome activation | - |
dc.subject.local | genomic imprinting | - |
dc.subject.local | Genomic imprinting | - |
dc.description.journalClass | Y | - |
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