Microfluidic device for one-step detection of breast cancer-derived exosomal mRNA in blood using signal-amplifiable 3D nanostructure = 혈액 내 유방암 유래 엑소좀 RNA 검출용 미세유체칩

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Title
Microfluidic device for one-step detection of breast cancer-derived exosomal mRNA in blood using signal-amplifiable 3D nanostructure = 혈액 내 유방암 유래 엑소좀 RNA 검출용 미세유체칩
Author(s)
Jaewoo Lim; Byunghoon Kang; H Y Son; B Mun; Y M Huh; H W Rho; Taejoon Kang; Jeong Moon; J J Lee; Seungbeom Seo; Soojin Jang; Seong Uk Son; Juyeon Jung; S Haam; Eun Kyung Lim
Bibliographic Citation
Biosensors & Bioelectronics, vol. 197, pp. 113753-113753
Publication Year
2022
Abstract
Metastasis attributed to approximately 90% of cancer-related deaths; hence, the detection of metastatic tumor-derived components in the blood assists in determining cancer recurrence and patient survival. Microfluidic-based sensors facilitate analysis of small fluid volumes and represent an accurate, rapid, and user-friendly method of field diagnoses. In this study, we have developed a microfluidic chip-based exosomal mRNA sensor (exoNA-sensing chip) for the one-step detection of exosomal ERBB2 in the blood by integrating a microfluidic chip and 3D-nanostructured hydrogels. The exoNA-sensing chip is a vacuum-driven power-free microfluidic chip that can accurately control the flow of trace fluids (<100 μL). The sensing part of the exoNA-sensing chip includes 3D-nanostructured hydrogels capable of detecting ERBB2 and a reference gene by amplifying a fluorescent signal via an enzyme-free catalytic hairpin assembly reaction at room temperature. This hydrogel offers a detection limit of 58.3 fM with good selectivity for target sequences. The performance of the exoNA-sensing chip was evaluated by testing in vitro and in vivo samples and was proven to be effective for cancer diagnosis and liquid biopsies.
Keyword
MicrofluidicsBiosensorsPoint-of-care detectionExosomal mRNAThree-dimensional-nanostructured hydrogels
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2021.113753
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
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