Lagerstroemia ovalifolia exerts anti-inflammatory effects in mice of LPS-induced ALI via downregulating of MAPK and NF-κB activation = 폐렴 마우스에서 라저스트로에미아 오바리폴리아 추출물의 항염증 효과

Abstract

It was reported that Lagerstroemia ovalifolia Teijsm. & Binn. (LO) has been used as Traditional herbal medicine (THM) in Java. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Based on this finding, the current study aimed to evaluate the protective effects of LOLE in a mouse model of LPS-induced acute lung injury (ALI). The results showed that treatment with LPS enhanced the inflammatory cell influx into the lungs and increased the number of macrophages and the secretion of the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) of mice. However, these effects were notably abrogated with LOLE pretreatment. Furthermore, the increase of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissues of mice with ALI was also reversed by LOLE. In addition, LOLE significantly suppressed the LPS-induced activation of the MAPK/NF-κB signaling pathway and led to heme oxygenase-1 (HO-1) induction in the lungs. Additionally, in vitro experiments showed that LOLE enhanced the expression of HO-1 in RAW264.7 macrophages. Collectively, the aforementioned findings indicated that LOLE exerted an ameliorative effect on inflammatory response in airway of ALI mice.