Adhesive antimicrobial peptides containing 3,4-dihydroxy-L-phenylalanine residues for direct one-step surface coating

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Title
Adhesive antimicrobial peptides containing 3,4-dihydroxy-L-phenylalanine residues for direct one-step surface coating
Author(s)
Y E Hwang; Seonghun Im; H Kim; Jung Hoon Sohn; B K Cho; J H Cho; Bong Hyun Sung; S C Kim
Bibliographic Citation
International Journal of Molecular Sciences, vol. 22, no. 21, pp. 11915-11915
Publication Year
2021
Abstract
Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent synthetic antimicrobial peptide, and the adhesiveness and antibacterial properties of the resulting peptides are evaluated. The peptide is successfully immobilized on polystyrene, titanium, and polydimethylsiloxane surfaces within 10 min in a one-step coating process with no prior surface functionalization. The antibacterial effectiveness of the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces is confirmed by complete inhibition of the growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus within 2 h. The stability of the peptide coated on the substrate surface is maintained for 84 days, as confirmed by its bactericidal activity. Additionally, the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces show no cytotoxicity toward the human keratinocyte cell line HaCaT. The antimicrobial properties of the peptide-coated surfaces are confirmed in a subcutaneous implantation animal model. The adhesive antimicrobial peptide developed in this study exhibits potential as an antimicrobial surface-coating agent for efficiently killing a broad spectrum of bacteria on contact.
Keyword
Antimicrobial peptideCoatingInfectionL-DOPAin vivo bactericidal activity
ISSN
1661-6596
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms222111915
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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