Caragana rosea Turcz methanol extract inhibits lipopolysaccharide-induced inflammatory responses by suppressing the TLR4/NF-κB/IRF3 signaling pathways

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Title
Caragana rosea Turcz methanol extract inhibits lipopolysaccharide-induced inflammatory responses by suppressing the TLR4/NF-κB/IRF3 signaling pathways
Author(s)
A Mitra; A Ahuja; L Rahmawati; H G Kim; B Y Woo; Y D Hong; M A Hossain; Z Zhang; Soo Yong KimJ Lee; J H Kim; J Y Cho
Bibliographic Citation
Molecules, vol. 26, no. 21, pp. 6660-6660
Publication Year
2021
Abstract
Caragana rosea Turcz, which belongs to the Leguminosae family, is a small shrub found in Northern and Eastern China that is known to possess anti-inflammatory properties and is used to treat fever, asthma, and cough. However, the underlying molecular mechanisms of its anti-inflammatory effects are unknown. Therefore, we used lipopolysaccharide (LPS) in RAW264.7 macrophages to investigate the molecular mechanisms that underlie the anti-inflammatory activities of a methanol extract of Caragana rosea (Cr-ME). We showed that Cr-ME reduced the production of nitric oxide (NO) and mRNA levels of iNOS, TNF-α, and IL-6 in a concentration-dependent manner. We also found that Cr-ME blocked MyD88- and TBK1-induced NF-κB and IRF3 promoter activity, suggesting that it affects multiple targets. Moreover, Cr-ME reduced the phosphorylation levels of IκBα, IKKα/β and IRF3 in a time-dependent manner and regulated the upstream NF-κB proteins Syk and Src, and the IRF3 protein TBK1. Upon overexpression of Src and TBK1, Cr-ME stimulation attenuated the phosphorylation of the NF-κB subunits p50 and p65 and IRF3 signaling. Together, our results suggest that the anti-inflammatory activity of Cr-ME occurs by inhibiting the NF-κB and IRF3 signaling pathways.
Keyword
Caragana rosea Turcz (Cr-ME)Anti-inflammatory activityNF-κB and IRF3 signaling pathways
ISSN
1420-3049
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/molecules26216660
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > International Biological Material Research Center > 1. Journal Articles
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