Faecalicatena faecalis sp. nov., a moderately alkaliphilic bacterial strain isolated from swine faeces

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Title
Faecalicatena faecalis sp. nov., a moderately alkaliphilic bacterial strain isolated from swine faeces
Author(s)
Byeong Seob Oh; Ji Sun Kim; Seoung Woo Ryu; Seungyeob Yu; Jung-Sook LeeSeung Hwan ParkSe Won KangJiyoung LeeMi Kyung LeeKang Hyun Lee; Won Yong Jung; H Jung; T Y Hur; H B Kim; J K Kim; J H Lee; J H Jeong; Ju Huck Lee
Bibliographic Citation
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 114, no. 12, pp. 2091-2099
Publication Year
2021
Abstract
An obligately anaerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped strain AGMB00832T was isolated from swine faeces. Phylogenetic analysis based on the 16S rRNA gene, together with the housekeeping genes, gyrB and rpoD, revealed that strain AGMB00832T belonged to the genus Faecalicatena and was most closely related to Faecalicatena orotica KCTC 15331T. In biochemical analysis, strain AGMB00832T was shown to be negative for catalase, oxidase and urease. Furthermore, the isolate was positive for β-glucosidase, β-glucuronidase, glutamic acid decarboxylase, proline arylamidase, acid phosphatase and naphthol-AS-BI-phosphohydrolase. The major cellular fatty acids (> 10%) of the isolate were C14:0, C16:0 and C18:1ω11t DMA. Based on the whole genome sequence analysis, the DNA G + C content of strain AGMB00832T was 44.2 mol%, and the genome size and numbers of rRNA and tRNA genes were 5,175,159 bp, 11 and 53, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between strain AGMB00832T and related strains were ≤ 77.4 and 22.5%, respectively. Furthermore, the genome analysis revealed the presence of genes for alkaline shock protein 23 and cation/proton antiporters, which may facilitate growth of strain AGMB00832T in alkaline culture condition. On the basis of polyphasic taxonomic approach, strain AGMB00832T represents a novel species within the genus Faecalicatena, for which the name Faecalicatena faecalis sp. nov. is proposed. The type strain is AGMB00832T (= KCTC 15946T = NBRC 114613T).
Keyword
Faecalicatena faecalis sp. nov.Gut microbiotaSwine faecesTaxonomy
ISSN
0003-6072
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10482-021-01663-y
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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