Identification and function analysis of bHLH genes in response to cold stress in sweetpotato

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dc.contributor.authorR Jin-
dc.contributor.authorHo Soo Kim-
dc.contributor.authorT Yu-
dc.contributor.authorA Zhang-
dc.contributor.authorY Yang-
dc.contributor.authorM Liu-
dc.contributor.authorW Yu-
dc.contributor.authorP Zhao-
dc.contributor.authorQ Zhang-
dc.contributor.authorQ Cao-
dc.contributor.authorSang Soo Kwak-
dc.contributor.authorZ Tang-
dc.date.accessioned2021-11-22T15:30:43Z-
dc.date.available2021-11-22T15:30:43Z-
dc.date.issued2021-
dc.identifier.issn0981-9428-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/25042-
dc.description.abstractBasic/helix-loop-helix (bHLH) transcription factors are involved in various metabolic and physiological processes in plants. Sweetpotato (Ipomoea batatas (L.) Lam.) is an important crop in China but is highly susceptible to cold stress. However, little information on the bHLH gene family is available, and the function of this family in response to cold stress has not been revealed in sweetpotato. Here, 110 IbbHLHs were identified and classified into 17 categories based on phylogenetic relationships, conserved motifs and gene structure analyses. Except for 5 IbbHLHs, 90 IbbHLHs were putative E-box-binding proteins including 70 IbbHLHs belonging to G-box, whereas 15 IbbHLHs were putative non-E box-binding proteins based on DNA-binding analysis. In total, 37 pairs of segmental duplicated genes and 5 pairs of tandem duplication genes were identified within the IbbHLH gene family. The transcript level of 20 IbbHLHs was regulated by cold stress based on RNA-seq data, and 8 genes were selected for further quantitative real-time PCR (qRT-PCR) analysis. IbHLH8 and IbHLH92 are involved in network interaction with several genes related to abiotic and biotic stresses under cold treatment. IbbHLH79, an ICE1-like gene, was isolated and overexpressed in sweetpotato. The IbbHLH79 protein can activate the CBF (C-repeat Binding Factor) pathway, and IbbHLH79-overexpressing transgenic plants display enhanced cold tolerance. Taken together, these results provide valuable information on the IbbHLH gene family; in addition, several IbbHLHs may regulate cold stress, and the results suggest IbbHLH79 will be useful for molecular breeding of enhanced cold tolerance in sweetpotato.-
dc.publisherElsevier-
dc.titleIdentification and function analysis of bHLH genes in response to cold stress in sweetpotato-
dc.title.alternativeIdentification and function analysis of bHLH genes in response to cold stress in sweetpotato-
dc.typeArticle-
dc.citation.titlePlant Physiology and Biochemistry-
dc.citation.number0-
dc.citation.endPage235-
dc.citation.startPage224-
dc.citation.volume169-
dc.contributor.affiliatedAuthorHo Soo Kim-
dc.contributor.affiliatedAuthorSang Soo Kwak-
dc.contributor.alternativeNameJin-
dc.contributor.alternativeName김호수-
dc.contributor.alternativeNameYu-
dc.contributor.alternativeNameZhang-
dc.contributor.alternativeNameYang-
dc.contributor.alternativeNameLiu-
dc.contributor.alternativeNameYu-
dc.contributor.alternativeNameZhao-
dc.contributor.alternativeNameZhang-
dc.contributor.alternativeNameCao-
dc.contributor.alternativeName곽상수-
dc.contributor.alternativeNameTang-
dc.identifier.bibliographicCitationPlant Physiology and Biochemistry, vol. 169, pp. 224-235-
dc.identifier.doi10.1016/j.plaphy.2021.11.027-
dc.subject.keywordSweetpotato-
dc.subject.keywordbHLH gene family-
dc.subject.keywordCold stress-
dc.subject.keywordICE1-Like gene-
dc.subject.localsweet potato-
dc.subject.localsweet potatoes-
dc.subject.localSweet potato (Ipomoea batatas L. Lam)-
dc.subject.localSweet potato (Ipomoea batatas)-
dc.subject.localSweet otato-
dc.subject.localsweet potato (Ipomoea batatas)-
dc.subject.localipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas)-
dc.subject.localsweetpotato-
dc.subject.localSweetpotato Ipomoea batatas-
dc.subject.localSweetpotato (Ipomoea batatas L.)-
dc.subject.localSweetpotato-
dc.subject.localSweetpotato (Ipomoea batatas (L.) Lam)-
dc.subject.localSweet potato-
dc.subject.localIpomoea batatas-
dc.subject.localSweet potato (Ipomoea batatas (L.) Lam.)-
dc.subject.localbHLH gene family-
dc.subject.localCold stress-
dc.subject.localICE1-Like gene-
dc.description.journalClassY-
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Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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