The potential role of human NME1 in neuronal differentiation of porcine mesenchymal stem cells: application of NB-hNME1 as a human NME1 suppressor
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- The potential role of human NME1 in neuronal differentiation of porcine mesenchymal stem cells: application of NB-hNME1 as a human NME1 suppressor
- J H Cho; W S Ju; S Y Seo; B H Kim; Ji-Su Kim; J G Kim; S J Park; Y K Choo
- Bibliographic Citation
- International Journal of Molecular Sciences, vol. 22, no. 22, pp. 12194-12194
- Publication Year
- This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.
- MacrophageMiniature pig adipose tissue-derived mesenchymal stem cellsNanobodyNucleoside diphosphate kinase AST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
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- Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
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