LW1497, an inhibitor of malate dehydrogenase, suppresses TGF-β1-induced epithelial-mesenchymal transition in lung cancer cells by downregulating slug

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dc.contributor.authorH J Kim-
dc.contributor.authorM K Park-
dc.contributor.authorH J Byun-
dc.contributor.authorM Kim-
dc.contributor.authorB Kim-
dc.contributor.authorL Yu-
dc.contributor.authorT M Nguyen-
dc.contributor.authorT H Nguyen-
dc.contributor.authorP A Do-
dc.contributor.authorE J Kim-
dc.contributor.authorJ H Kim-
dc.contributor.authorE Enkhtaivan-
dc.contributor.authorK S Kim-
dc.contributor.authorJ Y Jang-
dc.contributor.authorG J Kang-
dc.contributor.authorH Lee-
dc.contributor.authorMi Sun Won-
dc.contributor.authorK Lee-
dc.contributor.authorJ Cho-
dc.contributor.authorC H Lee-
dc.date.accessioned2021-11-30T15:30:33Z-
dc.date.available2021-11-30T15:30:33Z-
dc.date.issued2021-
dc.identifier.issn2076-3921-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/25062-
dc.description.abstractLW1497 suppresses the expression of the hypoxia-inducing factor (HIF)-1α inhibiting malate dehydrogenase. Although hypoxia and HIF-1α are known to be important in cancer, LW1497 has not been therapeutically applied to cancer yet. Thus, we investigated the effect of LW1497 on the epithelial-mesenchymal transition (EMT) of lung cancer cells. A549 and H1299 lung cancer cells were induced to undergo via TGF-β1 treatment, resulting in the downregulation of E-cadherin and upregulation of N-cadherin and Vimentin concurrently with increases in the migration and invasion capacities of the cells. These effects of TGF-β1 were suppressed upon co-treatment of the cells with LW1497. An RNA-seq analysis revealed that LW1497 induced differential expression of genes related to hypoxia, RNA splicing, angiogenesis, cell migration, and metastasis in the A549 lung cancer cell lines. We confirmed the differential expression of Slug, an EMT-related transcription factor. Results from Western blotting and RT-PCR confirmed that LW1497 inhibited the expression of EMT markers and Slug. After orthotopically transplanting A549 cancer cells into mice, LW1497 was administered to examine whether the lung cancer progression was inhibited. We observed that LW1497 reduced the area of cancer. In addition, the results from immunohistochemical analyses showed that LW1497 downregulated EMT markers and Slug. In conclusion, LW1497 suppresses cancer progression through the inhibition of EMT by downregulating Slug.-
dc.publisherMDPI-
dc.titleLW1497, an inhibitor of malate dehydrogenase, suppresses TGF-β1-induced epithelial-mesenchymal transition in lung cancer cells by downregulating slug-
dc.title.alternativeLW1497, an inhibitor of malate dehydrogenase, suppresses TGF-β1-induced epithelial-mesenchymal transition in lung cancer cells by downregulating slug-
dc.typeArticle-
dc.citation.titleAntioxidants-
dc.citation.number11-
dc.citation.endPage1674-
dc.citation.startPage1674-
dc.citation.volume10-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.alternativeName김현지-
dc.contributor.alternativeName박미경-
dc.contributor.alternativeName변현정-
dc.contributor.alternativeName김민경-
dc.contributor.alternativeName김보람-
dc.contributor.alternativeNameYu-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeNameDo-
dc.contributor.alternativeName김은지-
dc.contributor.alternativeName김지현-
dc.contributor.alternativeNameEnkhtaivan-
dc.contributor.alternativeName김경성-
dc.contributor.alternativeName장지윤-
dc.contributor.alternativeName강경진-
dc.contributor.alternativeName이호-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName이경-
dc.contributor.alternativeName조정숙-
dc.contributor.alternativeName이창훈-
dc.identifier.bibliographicCitationAntioxidants, vol. 10, no. 11, pp. 1674-1674-
dc.identifier.doi10.3390/antiox10111674-
dc.subject.keywordMalate dehydrogenase-
dc.subject.keywordEpithelial-mesenchymal transition-
dc.subject.keywordHypoxia inducible factor-α1-
dc.subject.keywordSlug-
dc.subject.keywordA549-
dc.subject.keywordLocal oxygen tension-
dc.subject.keywordLW1497-
dc.subject.localMalate dehydrogenase-
dc.subject.localmalate dehyfrogenase-
dc.subject.localmalate dehydrogenase-
dc.subject.localEpithelial-mesenchymal transition-
dc.subject.localEpithelial-mesenchymal transition (EMT)-
dc.subject.localEpithelial.mesenchymal transition-
dc.subject.localEpithelialmesenchymal transition-
dc.subject.localepithelial-mesenchymal transition-
dc.subject.localHypoxia inducible factor-α1-
dc.subject.localSlug-
dc.subject.localA549-
dc.subject.localLocal oxygen tension-
dc.subject.localLW1497-
dc.description.journalClassY-
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Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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