Phosphorylation of β-catenin Ser60 by polo-like kinase 1 drives the completion of cytokinesis

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Title
Phosphorylation of β-catenin Ser60 by polo-like kinase 1 drives the completion of cytokinesis
Author(s)
Ji Eun YuYu; Sun Ok Kim; J A Hwang; J T Hong; Joonsung HwangNak-Kyun SoungHyunjoo Cha-Molstad; Y T Kwon; Bo Yeon KimKyung Ho Lee
Bibliographic Citation
EMBO Reports, vol. 22, no. 12, pp. e51503-e51503
Publication Year
2021
Abstract
β-Catenin is a multifunctional protein and participates in numerous processes required for embryonic development, cell proliferation, and homeostasis through various molecular interactions and signaling pathways. To date, however, there is no direct evidence that β-catenin contributes to cytokinesis. Here, we identify a novel p-S60 epitope on β-catenin generated by Plk1 kinase activity, which can be found at the actomyosin contractile ring of early telophase cells and at the midbody of late telophase cells. Depletion of β-catenin leads to cytokinesis-defective phenotypes, which eventually result in apoptotic cell death. In addition, phosphorylation of β-catenin Ser60 by Plk1 is essential for the recruitment of Ect2 to the midbody, activation of RhoA, and interaction between β-catenin, Plk1, and Ect2. Time-lapse image analysis confirmed the importance of β-catenin phospho-Ser60 in furrow ingression and the completion of cytokinesis. Taken together, we propose that phosphorylation of β-catenin Ser60 by Plk1 in cooperation with Ect2 is essential for the completion of cytokinesis. These findings may provide fundamental knowledge for the research of cytokinesis failure-derived human diseases.
Keyword
CytokinesisEct2MidbodyPlk1beta-Catenin p-S60
ISSN
1469-221X
Publisher
Wiley
DOI
http://dx.doi.org/10.15252/embr.202051503
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Ochang Branch Institute > Nucleic Acid Therapeutics Research Center > 1. Journal Articles
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