Construction of an artificial biosynthetic pathway for zingerone production in Escherichia coli using benzalacetone synthase from Piper methysticum
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- Construction of an artificial biosynthetic pathway for zingerone production in Escherichia coli using benzalacetone synthase from Piper methysticum
- Kyung Taek Heo; Kyung Won Park; Ju Hee Won; Byeong San Lee; Jae-Hyuk Jang; Jungoh Ahn; B Y Hwang; Young-Soo Hong
- Bibliographic Citation
- Journal of Agricultural and Food Chemistry, vol. 69, no. 48, pp. 14620-14629
- Publication Year
- Zingerone (vanillylacetone; 4-hydroxy-3-methoxyphenylethyl methyl ketone) is a key component responsible for the pungency of ginger (Zingiber officinale). In this study, it was confirmed that a type III polyketide synthase (PKS) gene (pmpks) from Piper methysticum exhibits feruloyl-CoA-preferred benzalacetone synthase (BAS) activity. Based on these results, we constructed an artificial biosynthetic pathway for zingerone production from supplemented ferulic acid with 4-coumarate CoA ligase (4CL), PmPKS, and benzalacetone reductase (BAR). Furthermore, a de novo pathway for the production of zingerone was assembled using six heterologous genes, encoding tyrosine ammonia-lyase (optal), cinnamate-4-hydroxlase (sam5), caffeic acid O-methyltransferase (com), 4CL (4cl2nt), BAS (pmpks), and BAR (rzs1), in Escherichia coli. Using the engineered l-tyrosine-overproducing E. coli ΔCOS4 strain as a host, a maximum yield of 24.03 ± 2.53 mg/L zingerone was achieved by complete de novo synthesis.
- Zingerone; Benzalacetone synthase; Natural flavor; De novo biosynthesis; Type III PKS
- Amer Chem Soc
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- Ochang Branch Institute > Anticancer Agent Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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