The docosahexaenoic acid derivatives, diHEP-DPA and TH-DPA, synthesized via recombinant lipoxygenase, ameliorate disturbances in lipid metabolism and liver inflammation in high fat diet-fed mice = 신규 지질대사체에 의한 고지방식이 유도 대사성 지방간염 제어 효과

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Title
The docosahexaenoic acid derivatives, diHEP-DPA and TH-DPA, synthesized via recombinant lipoxygenase, ameliorate disturbances in lipid metabolism and liver inflammation in high fat diet-fed mice = 신규 지질대사체에 의한 고지방식이 유도 대사성 지방간염 제어 효과
Author(s)
Lifang Wang; H S Choi; Yan Su; Jung-Hyun Ju; Sun-Yeon Heo; Jong-Jae Yi; Back Rock Oh; Y S Jang; Jeong-Woo Seo
Bibliographic Citation
Life Sciences, vol. 291, pp. 120219-120219
Publication Year
2022
Abstract
7S,15R-Dihydroxy-16S,17S-epoxy-docosapentaenoic acid (diHEP-DPA) and 7S,15R,16S,17S-tetrahydroxy-docosapentaenoic acid (TH-DPA) are two novel lipid mediators derived from docosahexaenoic acid (DHA) that we previously synthesized via combined enzymatic and chemical reactions. In the present study, we investigated the effects of these compounds on disturbances in lipid metabolism and liver inflammation induced by a high fat diet (HFD) in mice. Male BALB/c mice were randomly divided into four groups (n = 10/group): controls, HFD only, HFD + diHEP-DPA, and HFD + TH-DPA. Mice in HFD + diHEP-DPA and HFD + TH-DPA groups were orally administered 20 μg/kg of diHEP-DPA or TH-DPA, respectively. Measurements of adipose accumulation and liver inflammation showed that both diHEP-DPA and TH-DPA decreased adipose tissue mass and liver color depth, as well as total cholesterol, triglycerides, and low-density lipoprotein-cholesterol in the serum of HFD-fed mice compared with mice in the HFD-only group, while elevating high-density lipoprotein-cholesterol. Both of them also decreased hepatic expression of genes encoding lipid synthesis-related proteins (PPARγ, SIRT1, SREBP-1c and FASN) and increased the expression of genes encoding proteins involved in lipid degradation (PPARα and CPT-1) in the liver. Western blotting and quantitative RT-PCR confirmed that diHEP-DPA or TH-DPA administration modulated the expression of inflammation-related genes (TNF-α and IL-6) and inhibited activation of the NF-κB signaling pathway in livers of HFD-fed mice. Taken together, our data indicate that diHEP-DPA and THDPA ameliorate liver inflammation and inhibit HFD-induced obesity in mice.
Keyword
7S,15R-dihydroxy-16S,17S-epoxydocosapentaenoic acid (diHEP-DPA)7S,15R,16S,17S-tetrahydroxydocosapentaenoic acid (TH-DPA)Lipid metabolismHigh fat diet-induced obesityNF-κB
ISSN
0024-3205
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.lfs.2021.120219
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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