Construction of SARS-CoV-2 virus-like particles in plant

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Title
Construction of SARS-CoV-2 virus-like particles in plant
Author(s)
Ki Beom Moon; Jae Heung Jeon; H Choi; Ji-Sun Park; Su-Jin Park; Hyo Jun LeeJeong Mee ParkHye Sun ChoJae Sun MoonHyunwoo Oh; S Kang; H S Mason; Suk Yoon KwonHyun-Soon Kim
Bibliographic Citation
Scientific Reports, vol. 12, pp. 1005-1005
Publication Year
2022
Abstract
The pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused a public health emergency, and research on the development of various types of vaccines is rapidly progressing at an unprecedented development speed internationally. Some vaccines have already been approved for emergency use and are being supplied to people around the world, but there are still many ongoing efforts to create new vaccines. Virus-like particles (VLPs) enable the construction of promising platforms in the field of vaccine development. Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. Plant-derived VLPs were purified by sedimentation through a sucrose cushion. The shape and size of plant-derived VLPs are similar to native SARS-CoV-2 VLPs without spike. Although the assembled VLPs do not have S protein spikes, they could be developed as formulations that can improve the immunogenicity of vaccines including S antigens, and further could be used as platforms that can carry S antigens of concern for various mutations.
ISSN
2045-2322
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/s41598-022-04883-y
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
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