Effects of 3'-isovaleryl-4'-senecioylkhellactone from Peucedanum japonicum Thunberg on PMA-stimulated inflammatory response in A549 human lung epithelial cells
Cited 1 time in
- Effects of 3'-isovaleryl-4'-senecioylkhellactone from Peucedanum japonicum Thunberg on PMA-stimulated inflammatory response in A549 human lung epithelial cells
- Dasul Hwang; Hyung Won Ryu; Ji Won Park; Jung Hee Kim; Doo-Young Kim; Jaehoon Oh; Ok-Kyoung Kwon; S B Han; Kyung Seop Ahn
- Bibliographic Citation
- Journal of Microbiology and Biotechnology, vol. 32, no. 1, pp. 81-90
- Publication Year
- Peucedanum japonicum Thunberg (PJT) has been used in traditional medicine to treat colds, coughs, fevers and other inflammatory diseases. The goal of this study was to investigate whether 3′-isovaleryl-4′-senecioylkhellactone (IVSK) from PJT has anti-inflammatory effects on lung epithelial cells. Anti-inflammatory effects of IVSK was evaluated using phorbol 12-myristate 13-acetate (PMA)-stimulated A549 cells and regular human lung epithelial cells as a reference. IVSK reduced the secretion of inflammatory mediators, interleukin (IL)-8 and monocyte chemoattractant protein-1 (MCP-1) and the mRNA expression of IL-6, IL-8, MCP-1 and IL-1β. Additionally, it inhibited the phosphorylation of IκB kinase (IKK), p65, Iκ-Bα and mitogen-activated protein kinases (MAPKs) p38, JNK, and ERK in A549 cells stimulated with PMA. Moreover, the binding affinity of activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) was significantly reduced in the luciferase assay, and nuclear translocation was markedly inhibited by IVSK in the immunocytochemistry. These findings indicate that IVSK can protect against inflammation through the AP-1 and NF-κB pathway and could possibly be used as a lead compound for the treatment of inflammatory lung diseases.
- 3′-isovaleryl-4′-senecioylkhellactone; Peucedanum japonicum Thunberg; Inflammation; NF-κB; AP-1
- Korea Soc-Assoc-Inst
- Appears in Collections:
- Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.