Fluorescent polypropylene nanoplastics for studying uptake, biodistribution, and excretion in zebrafish embryos

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Title
Fluorescent polypropylene nanoplastics for studying uptake, biodistribution, and excretion in zebrafish embryos
Author(s)
Wang Sik Lee; Hyunjung Kim; Yugyeong Sim; Taejoon KangJinyoung Jeong
Bibliographic Citation
ACS Omega, vol. 7, no. 2, pp. 2467-2473
Publication Year
2022
Abstract
Nanoplastics (NPs) are emerging environmental pollutants and are a significant concern for human health. The small size of NPs allows them to accumulate within and adversely affect various tissues by penetrating the gastrointestinal barrier. However, most toxicity studies on NPs have been based on commercial polystyrene nanoparticles. Among plastics, polypropylene (PP) is one of the most widely used, and it is continuously micronized in the environment. Although PP has high potential for forming NPs by weathering, little is known about the biological effects of polypropylene nanoplastics (PPNPs) due to a lack of particle models. Here, we present a simple and high-yield method for PPNP production by nonsolvent-induced phase separation. The synthesized PPNPs were spherical in shape, with an average diameter of 562.15 ± 118.47 nm and a high yield of over 84%. These PPNPs were fluorescently labeled by the combined swelling-diffusion method to study their biodistribution after exposure to developing zebrafish embryos (ZFEs). We found that the fluorescent PPNPs were internalized by ingestion, distributed in the intestine of developing ZFEs, and eventually excreted. This study will aid evaluations of the potential risks of environmentally relevant plastics at the nanoscale.
ISSN
2470-1343
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/acsomega.1c06779
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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