Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2

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dc.contributor.authorHyunjung Cha-
dc.contributor.authorHyeran Kim-
dc.contributor.authorY Joung-
dc.contributor.authorHyunju Kang-
dc.contributor.authorJeong Moon-
dc.contributor.authorHyowon Jang-
dc.contributor.authorS Park-
dc.contributor.authorHyung-Jun Kwon-
dc.contributor.authorIn Chul Lee-
dc.contributor.authorS Kim-
dc.contributor.authorD Yong-
dc.contributor.authorSun Woo Yoon-
dc.contributor.authorS G Park-
dc.contributor.authorKyeonghye Guk-
dc.contributor.authorEun Kyung Lim-
dc.contributor.authorH G Park-
dc.contributor.authorJ Choo-
dc.contributor.authorJuyeon Jung-
dc.contributor.authorTaejoon Kang-
dc.date.accessioned2022-01-27T15:30:30Z-
dc.date.available2022-01-27T15:30:30Z-
dc.date.issued2022-
dc.identifier.issn0956-5663-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/25352-
dc.description.abstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected humans worldwide for over a year now. Although various tests have been developed for the detection of SARS-CoV-2, advanced sensing methods are required for the diagnosis, screening, and surveillance of coronavirus disease 2019 (COVID-19). Here, we report a surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2. The selected antibody pair against the SARS-CoV-2 antigen, along with the magnetic beads, facilitates the accurate direct detection of the virus. The hollow Au NPs exhibit strong, reproducible SERS signals, allowing sensitive quantitative detection of SARS-CoV-2. This assay had detection limits of 2.56 fg/mL for the SARS-CoV-2 antigen and 3.4 plaque-forming units/mL for the SARS-CoV-2 lysates. Furthermore, it facilitated the identification of SARS-CoV-2 in human nasopharyngeal aspirates and diagnosis of COVID-19 within 30 min using a portable Raman device. Thus, this assay can be potentially used for the diagnosis and prevention of COVID-19.-
dc.publisherElsevier-
dc.titleSurface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2-
dc.title.alternativeSurface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2-
dc.typeArticle-
dc.citation.titleBiosensors & Bioelectronics-
dc.citation.number0-
dc.citation.endPage114008-
dc.citation.startPage114008-
dc.citation.volume202-
dc.contributor.affiliatedAuthorHyunjung Cha-
dc.contributor.affiliatedAuthorHyeran Kim-
dc.contributor.affiliatedAuthorHyunju Kang-
dc.contributor.affiliatedAuthorJeong Moon-
dc.contributor.affiliatedAuthorHyowon Jang-
dc.contributor.affiliatedAuthorHyung-Jun Kwon-
dc.contributor.affiliatedAuthorIn Chul Lee-
dc.contributor.affiliatedAuthorSun Woo Yoon-
dc.contributor.affiliatedAuthorKyeonghye Guk-
dc.contributor.affiliatedAuthorEun Kyung Lim-
dc.contributor.affiliatedAuthorJuyeon Jung-
dc.contributor.affiliatedAuthorTaejoon Kang-
dc.contributor.alternativeName차현정-
dc.contributor.alternativeName김혜란-
dc.contributor.alternativeName정연주-
dc.contributor.alternativeName강현주-
dc.contributor.alternativeName문정-
dc.contributor.alternativeName장효원-
dc.contributor.alternativeName박소현-
dc.contributor.alternativeName권형준-
dc.contributor.alternativeName이인철-
dc.contributor.alternativeName김선주-
dc.contributor.alternativeName용동은-
dc.contributor.alternativeName윤선우-
dc.contributor.alternativeName박성규-
dc.contributor.alternativeName국경혜-
dc.contributor.alternativeName임은경-
dc.contributor.alternativeName박현규-
dc.contributor.alternativeName주재범-
dc.contributor.alternativeName정주연-
dc.contributor.alternativeName강태준-
dc.identifier.bibliographicCitationBiosensors & Bioelectronics, vol. 202, pp. 114008-114008-
dc.identifier.doi10.1016/j.bios.2022.114008-
dc.subject.keywordAntibody-
dc.subject.keywordCoronavirus disease 2019-
dc.subject.keywordImmunoassay-
dc.subject.keywordNanoparticle-
dc.subject.keywordSurface-enhanced Raman scattering-
dc.subject.keywordSevere acute respiratory syndrome coronavirus 2-
dc.subject.localantibody-
dc.subject.localAntibody-
dc.subject.localAntibodies-
dc.subject.localCoronavirus disease 2019-
dc.subject.localImmunoassay-
dc.subject.localImmunoassays-
dc.subject.localimmunoassay-
dc.subject.localNanoparticles-
dc.subject.localNanoparticle-
dc.subject.localnanoparticle-
dc.subject.localSurface-enhanced Raman scattering-
dc.subject.localsurface-enhanced raman scattering-
dc.subject.localSurface-enhanced Raman Scattering-
dc.subject.localsurface-enhanced Raman scattering-
dc.subject.localSevere acute respiratory syndrome coronavirus 2-
dc.subject.localsevere acute respiratory syndrome coronavirus 2-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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