Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages

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Title
Anti-inflammatory and antioxidant activities of Sargassum horneri extract in RAW264.7 macrophages
Author(s)
M J Kim; H G Jo; C Ramakrishna; Seung Jae Lee; D S Lee; S H Cheong
Bibliographic Citation
Physical Activity and Nutrition, vol. 25, no. 4, pp. 45-53
Publication Year
2021
Abstract
Purpose: In this study, we investigated whether a 70% ethanolic (EtOH) extract of Sargassum horneri had antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated macrophage-like RAW 264.7 cells. Methods: The proximate composition, fatty acids, amino acids, and dietary fiber of S. horneri, various biologically active compounds, and antioxidant activity were analyzed. Results: The DPPH and ABTS free radical scavenging activities, as well as the reduction power, of the S. horneri extract used here were significantly increased in a concentration-dependent manner. This indicates that S. horneri contains bioactive compounds, such as phenols and flavonoids, that have excellent antioxidant activity. The cellular viability and metabolic activity results confirmed that the extract had no discernible toxicity at concentrations up to 100 μg/mL. The levels of nitrites and cytokines (PGE2, TNF-α and IL-6), which mediate pro-inflammatory effect, were significantly inhibited by treatment with either 50 or 100 μg/mL S. horneri extract, whereas that of IL-1β was significantly inhibited by treatment with 100 μg/mL of the extract. Similarly, the expression of iNOS and COX-2 proteins also decreased according to 50 or 100 μg/mL extract concentrations. NF-κB binding to DNA was also significantly inhibited by treatment with 100 μg/mL of extract. Conclusion: These results suggest that 70% EtOH extracts of S. horneri can relieve inflammation caused by disease or high intensity exercise.
Keyword
Sargassum horneriAntioxidant activityAnti-inflammatory effectsRAW264.7 cells
ISSN
2733-7545
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.20463/pan.2021.0025
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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