Overexpression of IbMPK3 increases low-temperature tolerance in transgenic sweetpotato

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Title
Overexpression of IbMPK3 increases low-temperature tolerance in transgenic sweetpotato
Author(s)
R Jin; Ho Soo Kim; T Yu; M Liu; W Yu; P Zhao; A Zhang; Q Zhang; Y Liu; Q Cao; Sang Soo Kwak; Z Tang
Bibliographic Citation
Plant Biotechnology Reports, vol. 16, pp. 91-100
Publication Year
2022
Abstract
Sweetpotato is an important crop that is very sensitive to low temperatures. Mitogen-activated protein kinase (MAPK) is involved in plant growth and development and is responsive to many environmental stresses. IbMPK3 is strongly regulated by low-temperature stress. We studied the function of IbMPK3 and how it may enhance the adaptability of sweetpotato plants to low-temperature stress. Transgenic sweetpotato plants overexpressing IbMPK3 were generated, and three transgenic lines with the highest expression level of IbMPK3 were used for low-temperature tests. Transgenic plants exposed to low-temperature stress had less damage associated with higher photosynthesis efficiency and less cell membrane damage. IbMPK3-overexpressing transgenic plants could modulate reactive oxygen species (ROS) metabolism with lower levels of O2? and H2O2 accumulation and higher enzymatic activities than WT plants. Transcript expression levels of some ROS-related genes (IbCAT, IbCu-ZnSOD, and IbCAT) and stress-responsive genes (IbP3B and IbCOR27) were significantly upregulated in transgenic plants compared to WT. These results indicate that IbMPK3 has an important role in sweetpotato response to low temperatures.
Keyword
Low-temperature stressOverexpressionIbMPK3Sweetpotato
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-021-00730-0
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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