Defect in cytosolic Neu2 sialidase abrogates lipid metabolism and impairs muscle function in vivo

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Title
Defect in cytosolic Neu2 sialidase abrogates lipid metabolism and impairs muscle function in vivo
Author(s)
M Oh; Dae In Ha; C Son; Jeong Gu Kang; H Hwang; Su Bin Moon; M Kim; Jihae Nam; Jung Soo Kim; S Y Song; Yong-Sam Kim; S Park; J S Yoo; Jeong Heon Ko; K Park
Bibliographic Citation
Scientific Reports, vol. 12, pp. 3216-3216
Publication Year
2022
Abstract
Sialic acid (SA) is present in glycoconjugates and important in cell-cell recognition, cell adhesion, and cell growth and as a receptor. Among the four mammalian sialidases, cytosolic NEU2 has a pivotal role in muscle and neuronal differentiation in vitro. However, its biological functions in vivo remain unclear due to its very low expression in humans. However, the presence of cytoplasmic glycoproteins, gangliosides, and lectins involved in cellular metabolism and glycan recognition has suggested the functional importance of cytosolic Neu2 sialidases. We generated a Neu2 knockout mouse model via CRISPR/Cas9-mediated genome engineering and analyzed the offspring littermates at different ages to investigate the in vivo function of cytosolic Neu2 sialidase. Surprisingly, knocking out the Neu2 gene in vivo abrogated overall lipid metabolism, impairing motor function and leading to diabetes. Consistent with these results, Neu2 knockout led to alterations in sialylated glycoproteins involved in lipid metabolism and muscle function, as shown by glycoproteomics analysis.
ISSN
2045-2322
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/s41598-022-07033-6
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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