DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tran Bac Le | - |
dc.contributor.author | H K Kim | - |
dc.contributor.author | Min Ju Ahn | - |
dc.contributor.author | M Zanin | - |
dc.contributor.author | V T Lo | - |
dc.contributor.author | S Ling | - |
dc.contributor.author | Z Jiang | - |
dc.contributor.author | Jung-Ah Kang | - |
dc.contributor.author | P K Bae | - |
dc.contributor.author | Y S Kim | - |
dc.contributor.author | S Kim | - |
dc.contributor.author | S S Wong | - |
dc.contributor.author | Dae Gwin Jeong | - |
dc.contributor.author | Sun Woo Yoon | - |
dc.date.accessioned | 2022-03-04T15:34:00Z | - |
dc.date.available | 2022-03-04T15:34:00Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 0304-8608 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/25529 | - |
dc.description.abstract | Coronavirus disease 2019 (COVID-19) is an acute respiratory infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Other coronaviruses (CoVs) can also infect humans, although the majority cause only mild respiratory symptoms. Because early diagnosis of SARS-CoV-2 is critical for preventing further transmission events and improving clinical outcomes, it is important to be able to distinguish SARS-CoV-2 from other SARS-related CoVs in respiratory samples. Therefore, we developed and evaluated a novel reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting the genes encoding the spike (S) and membrane (M) proteins to enable the rapid identification of SARS-CoV-2, including several new circulating variants and other emerging SARS-like CoVs. By analysis of in vitro-transcribed mRNA, we established multiplex RT-qPCR assays capable of detecting 5 × 10° copies/reaction. Using RNA extracted from cell culture supernatants, our multiple simultaneous SARS-CoV-2 assays had a limit of detection of 1 × 10° TCID50/mL and showed no cross-reaction with human CoVs or other respiratory viruses. We also validated our method using human clinical samples from patients with COVID-19 and healthy individuals, including nasal swab and sputum samples. This novel one-step multiplex RT-qPCR assay can be used to improve the laboratory diagnosis of human-pathogenic CoVs, including SARS-CoV-2, and may be useful for the identification of other SARS-like CoVs of zoonotic origin. | - |
dc.publisher | Springer | - |
dc.title | Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses | - |
dc.title.alternative | Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses | - |
dc.type | Article | - |
dc.citation.title | Archives of Virology | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 879 | - |
dc.citation.startPage | 871 | - |
dc.citation.volume | 167 | - |
dc.contributor.affiliatedAuthor | Tran Bac Le | - |
dc.contributor.affiliatedAuthor | Min Ju Ahn | - |
dc.contributor.affiliatedAuthor | Jung-Ah Kang | - |
dc.contributor.affiliatedAuthor | Dae Gwin Jeong | - |
dc.contributor.affiliatedAuthor | Sun Woo Yoon | - |
dc.contributor.alternativeName | 르트란박 | - |
dc.contributor.alternativeName | 김혜권 | - |
dc.contributor.alternativeName | 안민주 | - |
dc.contributor.alternativeName | Zanin | - |
dc.contributor.alternativeName | Lo | - |
dc.contributor.alternativeName | Ling | - |
dc.contributor.alternativeName | Jiang | - |
dc.contributor.alternativeName | 강정아 | - |
dc.contributor.alternativeName | 배판기 | - |
dc.contributor.alternativeName | 김연숙 | - |
dc.contributor.alternativeName | 김승택 | - |
dc.contributor.alternativeName | Wong | - |
dc.contributor.alternativeName | 정대균 | - |
dc.contributor.alternativeName | 윤선우 | - |
dc.identifier.bibliographicCitation | Archives of Virology, vol. 167, no. 3, pp. 871-879 | - |
dc.identifier.doi | 10.1007/s00705-022-05383-0 | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.