Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses

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dc.contributor.authorTran Bac Le-
dc.contributor.authorH K Kim-
dc.contributor.authorMin Ju Ahn-
dc.contributor.authorM Zanin-
dc.contributor.authorV T Lo-
dc.contributor.authorS Ling-
dc.contributor.authorZ Jiang-
dc.contributor.authorJung-Ah Kang-
dc.contributor.authorP K Bae-
dc.contributor.authorY S Kim-
dc.contributor.authorS Kim-
dc.contributor.authorS S Wong-
dc.contributor.authorDae Gwin Jeong-
dc.contributor.authorSun Woo Yoon-
dc.date.accessioned2022-03-04T15:34:00Z-
dc.date.available2022-03-04T15:34:00Z-
dc.date.issued2022-
dc.identifier.issn0304-8608-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/25529-
dc.description.abstractCoronavirus disease 2019 (COVID-19) is an acute respiratory infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Other coronaviruses (CoVs) can also infect humans, although the majority cause only mild respiratory symptoms. Because early diagnosis of SARS-CoV-2 is critical for preventing further transmission events and improving clinical outcomes, it is important to be able to distinguish SARS-CoV-2 from other SARS-related CoVs in respiratory samples. Therefore, we developed and evaluated a novel reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting the genes encoding the spike (S) and membrane (M) proteins to enable the rapid identification of SARS-CoV-2, including several new circulating variants and other emerging SARS-like CoVs. By analysis of in vitro-transcribed mRNA, we established multiplex RT-qPCR assays capable of detecting 5 × 10° copies/reaction. Using RNA extracted from cell culture supernatants, our multiple simultaneous SARS-CoV-2 assays had a limit of detection of 1 × 10° TCID50/mL and showed no cross-reaction with human CoVs or other respiratory viruses. We also validated our method using human clinical samples from patients with COVID-19 and healthy individuals, including nasal swab and sputum samples. This novel one-step multiplex RT-qPCR assay can be used to improve the laboratory diagnosis of human-pathogenic CoVs, including SARS-CoV-2, and may be useful for the identification of other SARS-like CoVs of zoonotic origin.-
dc.publisherSpringer-
dc.titleDiagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses-
dc.title.alternativeDiagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses-
dc.typeArticle-
dc.citation.titleArchives of Virology-
dc.citation.number3-
dc.citation.endPage879-
dc.citation.startPage871-
dc.citation.volume167-
dc.contributor.affiliatedAuthorTran Bac Le-
dc.contributor.affiliatedAuthorMin Ju Ahn-
dc.contributor.affiliatedAuthorJung-Ah Kang-
dc.contributor.affiliatedAuthorDae Gwin Jeong-
dc.contributor.affiliatedAuthorSun Woo Yoon-
dc.contributor.alternativeName르트란박-
dc.contributor.alternativeName김혜권-
dc.contributor.alternativeName안민주-
dc.contributor.alternativeNameZanin-
dc.contributor.alternativeNameLo-
dc.contributor.alternativeNameLing-
dc.contributor.alternativeNameJiang-
dc.contributor.alternativeName강정아-
dc.contributor.alternativeName배판기-
dc.contributor.alternativeName김연숙-
dc.contributor.alternativeName김승택-
dc.contributor.alternativeNameWong-
dc.contributor.alternativeName정대균-
dc.contributor.alternativeName윤선우-
dc.identifier.bibliographicCitationArchives of Virology, vol. 167, no. 3, pp. 871-879-
dc.identifier.doi10.1007/s00705-022-05383-0-
dc.description.journalClassY-
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Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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