ACC deaminase-producing Brevibacterium linens RS16 enhances heat-stress tolerance of rice (Oryza sativa L.)

Abstract

The rapid rise in global temperature has adverse effects on rice productivity. The lack of eminent resources for heat stress alleviation is threatening the agricultural sector. Heat stress alleviation by endophytic plant growth-promoting bacteria (PGPB) can be a sustainable and eco-friendly approach. The present study was conducted to check the colonization of Brevibacterium linens RS16 producing ACC (1-aminocyclopropane-1-carboxylate) deaminase in the rice endosphere and to characterize its efficiency in enhancing stress tolerance. The ethylene emission pathway, reactive oxygen species (ROS) concentrations, proline accumulation, expression of glutathione S-transferase (GST), and small heat shock proteins (sHSPs) were monitored at two different levels of heat stress (40°C and 45°C). Bacterial inoculation decreased ethylene emission levels by 26.9% and 24.4% in rice plants exposed to 40°C and 45°C, respectively, compared with the non-inoculated plants. B. linens RS16 also enhanced the expression profiles of glutathione S-transferase. The collective effect of GST expression profiles and decrease in ethylene emission due to bacterial ACC deaminase activity subsequently resulted in a decrease in ROS concentrations. Additionally, HSP16 and HSP26 increased expression in heat-stressed plants inoculated with B. linens RS16 resulted in enhanced stress tolerance (i.e., lesser proline accumulation) than non-inoculated plants. Hence, this study demonstrates the bacteria-mediated tolerance against heat stress by regulating the ethylene emission pathway and upregulating antioxidant enzymes and heat shock proteins.