Monoclonal antibody K312-based depletion of pluripotent cells from differentiated stem cell progeny prevents teratoma formation

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dc.contributor.authorJongjin Park-
dc.contributor.authorDong Gwang Lee-
dc.contributor.authorNa Geum Lee-
dc.contributor.authorMin-Gi Kwon-
dc.contributor.authorYeon Sung Son-
dc.contributor.authorMi-Young Son-
dc.contributor.authorKwang-Hee Bae-
dc.contributor.authorJangwook Lee-
dc.contributor.authorJong Gil Park-
dc.contributor.authorNam-Kyung Lee-
dc.contributor.authorJeong Ki Min-
dc.date.accessioned2022-04-01T15:32:00Z-
dc.date.available2022-04-01T15:32:00Z-
dc.date.issued2022-
dc.identifier.issn19766696-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/25685-
dc.description.abstractHuman pluripotent stem cells (PSCs) have been utilized as a promising source in regenerative medicine. However, the risk of teratoma formation that comes with residual undifferentiated PSCs in differentiated cell populations is most concerning in the clinical use of PSC derivatives. Here, we report that a monoclonal antibody (mAb) targeting PSCs could distinguish undifferentiated PSCs, with potential teratoma-forming activity, from differentiated PSC progeny. A panel of hybridomas generated from mouse immunization with H9 human embryonic stem cells (hESCs) was screened for ESC-specific binding using flow cytometry. A novel mAb, K312, was selected considering its high stem cell-binding activity, and this mAb could bind to several human induced pluripotent stem cells and PSC lines. Cell-binding activity of K312 was markedly decreased as hESCs were differentiated into embryoid bodies or by retinoic acid treatment. In addition, a cell population negatively isolated from undifferentiated or differentiated H9 hESCs via K312 targeting showed a significantly reduced expression of pluripotency markers, including Oct4 and Nanog. Furthermore, K312-based depletion of pluripotent cells from differentiated PSC progeny completely prevented teratoma formation. Therefore, our findings suggest that K312 is utilizable in improving stem cell transplantation safety by specifically distinguishing residual undifferentiated PSCs.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMonoclonal antibody K312-based depletion of pluripotent cells from differentiated stem cell progeny prevents teratoma formation-
dc.title.alternativeMonoclonal antibody K312-based depletion of pluripotent cells from differentiated stem cell progeny prevents teratoma formation-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number3-
dc.citation.endPage147-
dc.citation.startPage142-
dc.citation.volume55-
dc.contributor.affiliatedAuthorJongjin Park-
dc.contributor.affiliatedAuthorDong Gwang Lee-
dc.contributor.affiliatedAuthorNa Geum Lee-
dc.contributor.affiliatedAuthorMin-Gi Kwon-
dc.contributor.affiliatedAuthorYeon Sung Son-
dc.contributor.affiliatedAuthorMi-Young Son-
dc.contributor.affiliatedAuthorKwang-Hee Bae-
dc.contributor.affiliatedAuthorJangwook Lee-
dc.contributor.affiliatedAuthorJong Gil Park-
dc.contributor.affiliatedAuthorNam-Kyung Lee-
dc.contributor.affiliatedAuthorJeong Ki Min-
dc.contributor.alternativeName박종진-
dc.contributor.alternativeName이동광-
dc.contributor.alternativeName이나금-
dc.contributor.alternativeName권민기-
dc.contributor.alternativeName손연성-
dc.contributor.alternativeName손미영-
dc.contributor.alternativeName배광희-
dc.contributor.alternativeName이장욱-
dc.contributor.alternativeName박종길-
dc.contributor.alternativeName이남경-
dc.contributor.alternativeName민정기-
dc.identifier.bibliographicCitationBMB Reports, vol. 55, no. 3, pp. 142-147-
dc.identifier.doi10.5483/BMBRep.2022.55.3.090-
dc.subject.keywordCell-surface marker-
dc.subject.keywordMonoclonal antibody-
dc.subject.keywordPluripotent stem cells-
dc.subject.keywordStem cell differentiation-
dc.subject.keywordTeratoma-
dc.subject.localCell-surface marker-
dc.subject.localMonoclonal antibody (mAb)-
dc.subject.localMonoclonal antibodies-
dc.subject.localMonoclonal Antibodies-
dc.subject.localMonoclonal antibody-
dc.subject.localmonoclonal antibody-
dc.subject.localMonoclonal Antibody-
dc.subject.localmonoclonal antibodies-
dc.subject.localPluripotent stem cell-
dc.subject.localpluripotent stem cells-
dc.subject.localpluripotent stem cell-
dc.subject.localPluripotent stem cells-
dc.subject.localStem cell differentiation-
dc.subject.localTeratoma-
dc.subject.localteratoma-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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