Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
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Title
Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
Author(s)
E A Mordukhova; Jae Gu Pan
Bibliographic Citation
Heliyon, vol. 6, pp. e03891-e03891
Publication Year
2020
Abstract
A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. The pHT-FabL40 plasmid is stable in both gram-positive and gram-negative hosts with increased plasmid DNA yield in E. coli. ⓒ 2020 The AuthorsMicrobiology; Bacterial genetics; Pharmaceutical science; Molecular biology; Triclosan & FabL & shuttle vector & E.coli & B. subtilis.