Isothermal amplification-mediated lateral flow biosensors for in vitro diagnosis of gastric cancer-related microRNAs

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dc.contributor.authorSeung Beom Seo-
dc.contributor.authorJin-Seong Hwang-
dc.contributor.authorE Kim-
dc.contributor.authorK Kim-
dc.contributor.authorS Roh-
dc.contributor.authorG Lee-
dc.contributor.authorJaewoo Lim-
dc.contributor.authorByunghoon Kang-
dc.contributor.authorSoojin Jang-
dc.contributor.authorSeong Uk Son-
dc.contributor.authorTaejoon Kang-
dc.contributor.authorJuyeon Jung-
dc.contributor.authorJang Seong Kim-
dc.contributor.authorK Hur-
dc.contributor.authorTae-Su Han-
dc.contributor.authorEun Kyung Lim-
dc.date.accessioned2022-05-03T15:31:50Z-
dc.date.available2022-05-03T15:31:50Z-
dc.date.issued2022-
dc.identifier.issn0039-9140-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/25971-
dc.description.abstractMicroRNAs (miRNAs) are important diagnostic and prognostic biomarkers for various tumors. Currently, many diagnostic systems have been developed to detect miRNAs, but simple techniques for detecting miRNAs are still required. Recently, we reported that the expression of miRNA-135b is upregulated in gastric epithelial cells during gastric inflammation and carcinogenesis. Our aim was to develop an in vitro diagnostic platform to analyze the expression of gastric cancer-related biomarkers in the blood. The diagnostic platform comprised an isothermal amplification-based lateral flow biosensor (IA-LFB) that enables easy diagnosis of gastric cancer through visual observation. In this platform, trace amounts of biomarkers are isothermally amplified through rolling circle amplification (RCA), and the amplified product is grafted to the LFB. The performance of the IA-LFB was confirmed using RNAs extracted from in vitro and in vivo models. The platform could detect target miRNAs within 3 h with excellent sensitivity and selectivity. In particular, the IA-LFB could detect the overexpression of gastric cancer-related markers (miRNA-135b and miRNA-21) in RNAs extracted from the blood of patients with various stages (stages 1?4) of gastric cancer compared to that in healthy volunteers. Therefore, IA-LFB is a simple and sensitive in vitro diagnostic system for detecting gastric cancer-related biomarkers and can contribute to the early diagnosis and prognosis monitoring of gastric cancer. Furthermore, this technology can be applied to systems that can detect multiple biomarkers related to various diseases (such as infectious and genetic diseases).-
dc.publisherElsevier-
dc.titleIsothermal amplification-mediated lateral flow biosensors for in vitro diagnosis of gastric cancer-related microRNAs-
dc.title.alternativeIsothermal amplification-mediated lateral flow biosensors for in vitro diagnosis of gastric cancer-related microRNAs-
dc.typeArticle-
dc.citation.titleTalanta-
dc.citation.number0-
dc.citation.endPage123502-
dc.citation.startPage123502-
dc.citation.volume246-
dc.contributor.affiliatedAuthorSeung Beom Seo-
dc.contributor.affiliatedAuthorJin-Seong Hwang-
dc.contributor.affiliatedAuthorJaewoo Lim-
dc.contributor.affiliatedAuthorByunghoon Kang-
dc.contributor.affiliatedAuthorSoojin Jang-
dc.contributor.affiliatedAuthorSeong Uk Son-
dc.contributor.affiliatedAuthorTaejoon Kang-
dc.contributor.affiliatedAuthorJuyeon Jung-
dc.contributor.affiliatedAuthorJang Seong Kim-
dc.contributor.affiliatedAuthorTae-Su Han-
dc.contributor.affiliatedAuthorEun Kyung Lim-
dc.contributor.alternativeName서승범-
dc.contributor.alternativeName황진성-
dc.contributor.alternativeName김은정-
dc.contributor.alternativeName김규정-
dc.contributor.alternativeName노석범-
dc.contributor.alternativeName이규도-
dc.contributor.alternativeName임재우-
dc.contributor.alternativeName강병훈-
dc.contributor.alternativeName장수진-
dc.contributor.alternativeName손성욱-
dc.contributor.alternativeName강태준-
dc.contributor.alternativeName정주연-
dc.contributor.alternativeName김장성-
dc.contributor.alternativeName허근-
dc.contributor.alternativeName한태수-
dc.contributor.alternativeName임은경-
dc.identifier.bibliographicCitationTalanta, vol. 246, pp. 123502-123502-
dc.identifier.doi10.1016/j.talanta.2022.123502-
dc.subject.keywordGastric cancer-
dc.subject.keywordmicroRNAs-
dc.subject.keywordLateral flow biosensor-
dc.subject.keywordRolling circle amplification-
dc.subject.keywordIn vitro diagnosis-
dc.subject.localGastric cancer-
dc.subject.localGastric cancer (GC)-
dc.subject.localgastric cancer-
dc.subject.localmicroRNA-
dc.subject.localmicroRNA (miRNA)-
dc.subject.localmicroRNAs-
dc.subject.localMicroRNA-
dc.subject.localMicroRNAs-
dc.subject.localmicro-RNA-
dc.subject.localMicroRNA.-
dc.subject.localLateral flow biosensor-
dc.subject.localRolling circle amplification-
dc.subject.localin-vitro diagnosis-
dc.subject.localIn vitro diagnosis-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
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