Identification, in silico characterization and differential expression profiles of carotenoid, xanthophyll, apocarotenoid biosynthetic pathways genes and analysis of carotenoid and xanthophyll accumulation in Heracleum moellendorffii hance

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Title
Identification, in silico characterization and differential expression profiles of carotenoid, xanthophyll, apocarotenoid biosynthetic pathways genes and analysis of carotenoid and xanthophyll accumulation in Heracleum moellendorffii hance
Author(s)
R Sathasivam; Nam Su Kim; M Choi; H Kwon; B V Nguyen; J K Kim; D H Jeong; E J Park; H W Park; S U Park
Bibliographic Citation
International Journal of Molecular Sciences, vol. 23, no. 9, pp. 4845-4845
Publication Year
2022
Abstract
Heracleum moellendorffii Hance is a non?woody forest plant widely used in China, Korea, and Japan because of its various therapeutic properties. However, the genetic details of the carote-noid pathway (CP), xanthophyll pathway (XP), and apocarotenoid pathway (AP) genes have not been studied. Thus, the CP, XP, and AP genes of H. moellendorffii were detected and analyzed. A total of fifteen genes were identified, of which eight, four, and three belonged to CP, XP, and AP, respectively. All identified genes possessed full open reading frames. Phylogenetic characterization of the identified gene sequences showed the highest similarity with other higher plants. Multiple alignments and 3D dimensional structures showed several diverse conserved motifs, such as the carotene?binding motif, dinucleotide?binding motif, and aspartate or glutamate residues. The results of real?time PCR showed that the CP, XP, and AP genes were highly expressed in leaves, followed by the stems and roots. In total, eight different individual carotenoids were identified using HPLC analysis. The highest individual and total carotenoid content were achieved in the leaves, followed by the stems and roots. This study will provide more information on the gene structure of the CP, XP, and AP genes, which may help to increase the accumulation of carotenoids in H. moel-lendorffii through genetic engineering. These results could be helpful for further molecular and functional studies of CP, XP, and AP genes.
Keyword
Heracleum moellendorffii HanceCarotenoid pathway genesXanthophyll pathway genesApocarotenoid pathway genesGene expressionCarotenoidXanthophyll
ISSN
1661-6596
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/ijms23094845
Type
Article
Appears in Collections:
National Research Safety Headquarter > 1. Journal Articles
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