Over-expression of dehydroascorbate reductase improves salt tolerance, environmental adaptability and productivity in Oryza sativa

Cited 6 time in scopus
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Title
Over-expression of dehydroascorbate reductase improves salt tolerance, environmental adaptability and productivity in Oryza sativa
Author(s)
Y S Kim; S I Park; J J Kim; S Y Shin; Sang Soo Kwak; C H Lee; H M Park; Y H Kim; I S Kim; H S Yoon
Bibliographic Citation
Antioxidants, vol. 11, no. 6, pp. 1077-1077
Publication Year
2022
Abstract
Abiotic stress induces reactive oxygen species (ROS) generation in plants, and high ROS levels can cause partial or severe oxidative damage to cellular components that regulate the redox status. Here, we developed salt-tolerant transgenic rice plants that overexpressed the dehydroascorbate reductase gene (OsDHAR1) under the control of a stress-inducible sweet potato promoter (SWPA2). OsDHAR1-expressing transgenic plants exhibited improved environmental adaptability compared to wild-type plants, owing to enhanced ascorbate levels, redox homeostasis, photosynthetic ability, and membrane stability through cross-activation of ascorbate-glutathione cycle enzymes under paddy-field conditions, which enhanced various agronomic traits, including root development, panicle number, spikelet number per panicle, and total grain yield. dhar2-knockdown plants were susceptible to salt stress, and owing to poor seed maturation, exhibited reduced biomass (root growth) and grain yield under paddy field conditions. Microarray revealed that transgenic plants highly expressed genes associated with cell growth, plant growth, leaf senescence, root development, ROS and heavy metal detoxification systems, lipid metabolism, isoflavone and ascorbate recycling, and photosynthesis. We identified the genetic source of functional genomics-based molecular breeding in crop plants and provided new insights into the physiological processes underlying environmental adaptability, which will enable improvement of stress tolerance and crop species productivity in response to climate change.
Keyword
AscorbateAscorbate-glutathione systemDehydroascorbate reductaseReactive oxygen speciesYeld parameters
ISSN
2076-3921
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/antiox11061077
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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