Antioxidative role of Hygrophila erecta (Brum. F.) Hochr. on UV-induced photoaging of dermal fibroblasts and melanoma cells

Cited 3 time in scopus
Metadata Downloads
Antioxidative role of Hygrophila erecta (Brum. F.) Hochr. on UV-induced photoaging of dermal fibroblasts and melanoma cells
S J Lee; J E Kim; Y J Choi; Y J Jin; Y J Roh; A Y Seol; H J Song; S H Park; M S Uddin; Sang Woo Lee; D Y Hwang
Bibliographic Citation
Antioxidants, vol. 11, no. 7, pp. 1317-1317
Publication Year
Antioxidants are an important strategy for treating photoaging because excessive reactive oxygen species (ROS) are produced during UV irradiation. The therapeutic effects of methanol extracts of Hygrophila erecta (Brum. F.) Hochr. (MEH) against UV-induced photoaging were examined by monitoring the changes in the antioxidant defense system, apoptosis, extracellular matrix (ECM) modulation, inflammatory response, and melanin synthesis in normal human dermal fibroblast (NHDF) cells and melanoma B16F1 cells. Four bioactive compounds, including 4-methoxycinnamic acid, 4-methoxybenzoic acid, methyl linoleate, and asterriquinone C-1, were detected in MEH, while the DPPH free radical scavenging activity was IC50 = 7.6769 μg/mL. UV-induced an increase in the intracellular ROS generation, NO concentration, SOD activity and expression, and Nrf2 expression were prevented with the MEH treatment. Significant decreases in the number of apoptotic cells, the ratio of Bax/Bcl-2, and cleaved Cas-3/Cas-3 were observed in MEH-treated NHDF cells. The MEH treatment induced the significant prevention of ECM disruption and suppressed the COX-2-induced iNOS mediated pathway, expression of inflammatory cytokines, and inflammasome activation. Finally, the expression of the melanin synthesis-involved genes and tyrosinase activity decreased significantly in the α-melanocyte-stimulating hormone (MSH)-stimulated B16F1 cells after the MEH treatment. MEH may have an antioxidative role against UV-induced photoaging by suppressing ROS-induced cellular damage.
Hygrophila erectaPhotoagingROSAntioxidantInflammationECMMelanin
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > International Biological Material Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.