Humibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from activated sludge

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dc.contributor.authorG M Choi-
dc.contributor.authorY W Lee-
dc.contributor.authorHanna Choe-
dc.contributor.authorSo Yeon Kim-
dc.contributor.authorW T Im-
dc.description.abstractA novel Gram-stain-positive, thin rod-shaped, non-motile, aerobic and creamy-white pigmented bacterium (designated strain WJ7-1T) was isolated from activated sludge sampled in Suwon, Republic of Korea. 16S rRNA gene sequence analysis indicated that the isolate belongs to the genus Humibacter, family Microbacteriaceae, with a sequence similarity of 97.9?% to its nearest neighbour Humibacter albus KACC 20986T. Strain WJ7-1T contained menaquinone (MK)-10 (16.0?%), 11 (48.5?%) and 12 (35.5?%) as major respiratory quinones. The predominant cellular fatty acids (>15?%) were anteiso-C17?:?0, iso-C16?:?0 and anteiso-C15?:?0. The peptidoglycan of strain WJ7-1T contained the diagnostic diamino acid ornithine and 2,4-diaminobutyric acid alanine. Alanine, glutamic acid and glycine were also present in the cell wall. The characteristic whole-cell sugars present were glucose, galactose, xylose and rhamnose. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and two unidentified phospholipids. Strain WJ7-1T possessed ginsenoside-converting (β-glucosidase) activity, which enabled it to transform ginsenoside protopanaxadiol-type Rb1 (one of the dominant active components of ginseng) to compound K. The genome size of strain WJ7-1T has 4.2 Mbp and the G+C content is 68.3?mol%. Average nucleotide identity, amino acid identity and digital DNA?DNA hybridization values between strain WJ7-1T and the closely related strain were 79.8, 36.1 and 23.5?%, respectively, indicating that strain WJ7-1T represents a novel species of the genus Humibacter. Strain WJ7-1T could be distinguished from the other members of the genus Humibacter by a number of chemotaxonomic and phenotypic characteristics. Based on polyphasic taxonomic analysis, strain WJ7-1T (=KACC 19729T=LMG 30802T) represents a novel species within the genus Humibacter, for which the name Humibacter ginsenosidimutans sp. nov. is proposed.-
dc.publisherMicrobiology Soc-
dc.titleHumibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from activated sludge-
dc.title.alternativeHumibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from activated sludge-
dc.citation.titleInternational Journal of Systematic and Evolutionary Microbiology-
dc.contributor.affiliatedAuthorHanna Choe-
dc.contributor.affiliatedAuthorSo Yeon Kim-
dc.identifier.bibliographicCitationInternational Journal of Systematic and Evolutionary Microbiology, vol. 72, no. 7, pp. 005442-005442-
dc.subject.keywordHumibacter ginsenosidimutans-
dc.subject.keywordPolyphasic taxonomy-
dc.subject.keyword16S rRNA gene sequence-
dc.subject.keywordGenome sequence-
dc.subject.keywordBioconversion of ginsenoside-
dc.subject.localHumibacter ginsenosidimutans-
dc.subject.localPolyphasic taxonomy-
dc.subject.localpolyphasic taxomony-
dc.subject.localpolyphasic taxonomy-
dc.subject.local16S rRNA gene sequence-
dc.subject.local16S rRNA gene sequencing-
dc.subject.localGenome sequence-
dc.subject.localgenome sequence-
dc.subject.localBioconversion of ginsenoside-
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