Biochemical characterization of fast- and slow-growing Rhizobium japonicum = Fast-growing과 slow-growing Rhizobium japonicum의 생화학적 특성

Abstract

Fast-growing, acid-producing soybean rhizobia were examined to determine their biochemical relatedness to each other, to typical slow-growing Rhizobium japonicum strains, and to other fast-growing species of Rhizobium. Although both the fast- and slow-growing soybean rhizobia were positive for catalase, urease, oxidase, nitrate reductase, and penicillinase, the fast-growing strains grouped with other fast-growing species of Rhizobium in that they tolerated 2% NaCl, were capable of growth at pH 9.5, utilized a large variety of carbohydrates (notably disaccharides), and produced serum zones in litmus milk. In addition, these fastgrowing strains were similar to other fast-growing species of Rhizobium in that they produced appreciable levels of P-galactosidase and nicotinamide adenine dinucleotide phosphate-linked 6-phosphogluconate dehydrogenase but had no detectable hydrogenase activity. The fast-growing soybean rhizobia share symbiotic host specificity with Brudyvhizobium japonicum, but appear to be related biochemically to the other fast-growing species of Rhizobium.