Glycine effect on spheroplasting and nodule bacteroids of Rhizobium japonicum = Rhizobium japonicum 원형질체 형성과 근류 bacteroids에 미치는 glycine의 영향

Abstract

Different spheroplasting methods using glycine were tried to fast and slow growing R. japonicum. Although one of the fast growers, R-271 showed normal growth in the presence of 4mg/ml glycine, cell morphology and colony forming unit (CFU) were greatly different from the cells of late log phase grown in the medium without glycine. In parallel, R-271 became sensitive to lysozyme after 6hr incubation in medium containing glycine (3.5mg/ml). After 24hr cultivation in glycine (100 micro g/ml) medium, one of the slow growers, R-214 was also susceptible to lysozyme action. Spheroplasting frequency of both strains was over 96 % by glycine and lysozyme