Selection of R. japonicum strains for developing soybean inoculant and plasmid characterization = 대두접종제 개발을 위한 우수근류균의 선발 및 plasmid 특성

Abstract

590 strains of Rhitobia were isolated from root nodules of the legumes coilecied at 223 sites in Korea. According to their host specificities they were classified into R. japonicun (218 strains), R. phaseoli (101 strains), R.trifolii(97 strains), I之- meliloti (4 strains), R. Ieguminosarium(、Y strain), Rhizobium species(101 strains), and unidentified species(159 strains). 3 potent strains R-138, R-168, and R--214 of R. japonicum have been selected based on the infectivity to soybean cultivar and efficiency of nitrogen fixation. It was observed that the fast-growing strains of R. japonicum contained 1 to 4 plasmids of M.W. of 35-300 Md. However, plasmids v/ere hardly detected for the slow-growing strains.