Evolution of molecular hydrogen from glucose by Rhodopseudomonas sp. KCTC 1437 = Rhodopseudomonas sp. KCTC 1437에 의한 포도당으로부터의 수소 생성

Abstract

Rhodopseudomonas sp. KCTC 1437 evolved molecular hydrogen efficiently under light illuminated anaerobic culture conditon in the presence of organic acids and various sugars, especially glucose when low concentration of NH_4 + of L-glutamate was added to cultures. it was revealed that hydrogen formation from Rhodopseudomonas sp. KCTC 1437 was mediated by two different enzyme systems. Under the nitrogen limiting condition, hydrogen evolution from glucose was catalyzed by nitrogenase. For the nitrogenase activation in vivo. the precultured cells grown on limiting concentration of NH_4^+ as a sole nitrogen source showed more capacity of hydrogen evolution from glucose in the presence of L-glutamate than any other cells grown on sufficient concentration of NH_4^+, L-glutamate NH_4^+, or both of L-glutamate and n_2. A significant volume of molecular hydrogen was evolved from glucose even in the presence of excess NH_4^+ either in the light or dark anaerobic condition, presumably due to the mediation of hydrogen evolution by fromic hydrogenlyase.